Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xanthomonas campestris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Xanthomonas campestris | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol | Xanthomonas campestris | the enzyme is involved in the biosyntesis of xanthan | UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | Q8GCH2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + 6-O-acetyl-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | GumK shows diminished activity with 6-O-acetyl-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol, a putative intermediate in the synthesis of xanthan. This indicates that acetylation of the internal mannose takes place after the formation of the GumK product | Xanthomonas campestris | UDP + GlcA-beta-(1->2)-(6-O-acetyl)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | - |
? | |
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | the enzyme does not show specificity for the lipidic portion of the acceptor. GumK shows diminished activity with 6-O-acetyl-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol, a putative intermediate in the synthesis of xanthan. This indicates that acetylation of the internal mannose takes place after the formation of the GumK product | Xanthomonas campestris | UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | - |
? | |
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol | the enzyme is involved in the biosyntesis of xanthan | Xanthomonas campestris | UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Xanthomonas campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Xanthomonas campestris |