Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, crystal structure of apo-enzyme at 1.9 A resolution, and of enzyme in complex with UDP, at 2.28 A resolution. Residue Asp157 serves as the general base in the transfer reaction. Residues M231, M273, E272, Y292, M306, K307, and Q310 interact with UDP. Cocrystallisation of GumK or mutant D157A in presence of UDP-glucuronate is not possible | Xanthomonas campestris |
Protein Variants | Comment | Organism |
---|---|---|
D157A | mutation in UDP binding site, loss of activity | Xanthomonas campestris |
D157E | mutation in UDP binding site, loss of activity | Xanthomonas campestris |
D157N | mutation in UDP binding site, loss of activity | Xanthomonas campestris |
D207A | mutation in UDP binding site, kcat/Km for UDP-glucuronate is identical to wild-type value | Xanthomonas campestris |
D234A | mutation in UDP binding site, kcat/Km for UDP-glucuronate is identical to wild-type value | Xanthomonas campestris |
E192A | mutation in UDP binding site, kcat/Km for UDP-glucuronate is identical to wild-type value | Xanthomonas campestris |
E272A | mutation in UDP binding site, kcat/KM is 4.6fold lower than wild-type value | Xanthomonas campestris |
E272D | mutation in UDP binding site, kcat/KM is 2fold lower than wild-type value | Xanthomonas campestris |
K307A | mutation in UDP binding site, kcat/KM is 560fold lower than wild-type value | Xanthomonas campestris |
M231A | mutation in UDP binding site, kcat/Km for UDP-glucuronate is similar to wild-type value | Xanthomonas campestris |
Q310A | mutation in UDP binding site, kcat/KM is 22.2fold lower than wild-type value | Xanthomonas campestris |
Y292A | mutation in UDP binding site, kcat/KM is 14.7fold lower than wild-type value | Xanthomonas campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
UDP-glucuronate | mutant D207A, pH 8.2, 20°C | Xanthomonas campestris | |
0.061 | - |
UDP-glucuronate | mutant E192A, pH 8.2, 20°C | Xanthomonas campestris | |
0.062 | - |
UDP-glucuronate | wild-type, pH 8.2, 20°C | Xanthomonas campestris | |
0.063 | - |
UDP-glucuronate | mutant D234A, pH 8.2, 20°C | Xanthomonas campestris | |
0.063 | - |
UDP-glucuronate | mutant M231A, pH 8.2, 20°C | Xanthomonas campestris | |
0.1 | - |
UDP-glucuronate | mutant E272D, pH 8.2, 20°C | Xanthomonas campestris | |
0.125 | - |
UDP-glucuronate | mutant E272A, pH 8.2, 20°C | Xanthomonas campestris | |
0.194 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant D207A, pH 8.2, 20°C | Xanthomonas campestris | |
0.198 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | wild-type, pH 8.2, 20°C | Xanthomonas campestris | |
0.199 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant E192A, pH 8.2, 20°C | Xanthomonas campestris | |
0.205 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant D234A, pH 8.2, 20°C | Xanthomonas campestris | |
0.21 | - |
UDP-glucuronate | mutant Y292A, pH 8.2, 20°C | Xanthomonas campestris | |
0.218 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant M231A, pH 8.2, 20°C | Xanthomonas campestris | |
0.246 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant Q310A, pH 8.2, 20°C | Xanthomonas campestris | |
0.267 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant E272D, pH 8.2, 20°C | Xanthomonas campestris | |
0.274 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant Y292A, pH 8.2, 20°C | Xanthomonas campestris | |
0.291 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant E272A, pH 8.2, 20°C | Xanthomonas campestris | |
0.39 | - |
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | mutant K307A, pH 8.2, 20°C | Xanthomonas campestris | |
0.44 | - |
UDP-glucuronate | mutant K307A, pH 8.2, 20°C | Xanthomonas campestris | |
1.19 | - |
UDP-glucuronate | mutant Q310A, pH 8.2, 20°C | Xanthomonas campestris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Xanthomonas campestris | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | Xanthomonas campestris | the enzyme is involved in biosynthesis of xanthan | UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | Q8GCH2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | - |
Xanthomonas campestris | UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | - |
? | |
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | the enzyme is involved in biosynthesis of xanthan | Xanthomonas campestris | UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gumK | - |
Xanthomonas campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
UDP-glucuronate | mutant K307A, pH 8.2, 20°C | Xanthomonas campestris | |
0.02 | - |
UDP-glucuronate | mutant Y292A, pH 8.2, 20°C | Xanthomonas campestris | |
0.038 | - |
UDP-glucuronate | mutant E272A, pH 8.2, 20°C | Xanthomonas campestris | |
0.072 | - |
UDP-glucuronate | mutant E272D, pH 8.2, 20°C | Xanthomonas campestris | |
0.075 | - |
UDP-glucuronate | mutant Q310A, pH 8.2, 20°C | Xanthomonas campestris | |
0.084 | - |
UDP-glucuronate | mutant D207A, pH 8.2, 20°C | Xanthomonas campestris | |
0.087 | - |
UDP-glucuronate | mutant E192A, pH 8.2, 20°C | Xanthomonas campestris | |
0.087 | - |
UDP-glucuronate | mutant M231A, pH 8.2, 20°C | Xanthomonas campestris | |
0.087 | - |
UDP-glucuronate | wild-type, pH 8.2, 20°C | Xanthomonas campestris | |
0.088 | - |
UDP-glucuronate | mutant D234A, pH 8.2, 20°C | Xanthomonas campestris |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of xanthan | Xanthomonas campestris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
UDP-glucuronate | mutant K307A, pH 8.2, 20°C | Xanthomonas campestris | |
0.063 | - |
UDP-glucuronate | mutant Q310A, pH 8.2, 20°C | Xanthomonas campestris | |
0.095 | - |
UDP-glucuronate | mutant Y292A, pH 8.2, 20°C | Xanthomonas campestris | |
0.304 | - |
UDP-glucuronate | mutant E272A, pH 8.2, 20°C | Xanthomonas campestris | |
0.72 | - |
UDP-glucuronate | mutant E272D, pH 8.2, 20°C | Xanthomonas campestris | |
1.28 | - |
UDP-glucuronate | mutant M231A, pH 8.2, 20°C | Xanthomonas campestris | |
1.4 | - |
UDP-glucuronate | mutant D207A, pH 8.2, 20°C | Xanthomonas campestris | |
1.4 | - |
UDP-glucuronate | mutant D234A, pH 8.2, 20°C | Xanthomonas campestris | |
1.4 | - |
UDP-glucuronate | wild-type, pH 8.2, 20°C | Xanthomonas campestris | |
1.42 | - |
UDP-glucuronate | mutant E192A, pH 8.2, 20°C | Xanthomonas campestris |