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Literature summary for 2.4.1.27 extracted from

  • Lariviere, L.; Morera, S.
    A base-flipping mechanism for the T4 phage beta-glucosyltransferase and identification of a transition-state analog (2002), J. Mol. Biol., 324, 483-490.
    View publication on PubMed
Search for more literature for 2.4.1.27

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of the enzyme with UDPglucose and a 13mer DNA fragment containing an abasic site. Two crystal structures are obtained of a ternary complex BGT-UDP-DNA at 1.8 A and 2.5 A resolution, one with a Tris molecule and the other with a metal ion at the active site Tequatrovirus T4
ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA Tequatrovirus T4 the enzyme modifies T4-DNA UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P04547
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Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA base-flipping mechanism Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
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 Tequatrovirus T4 UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA the enzyme modifies T4-DNA Tequatrovirus T4 UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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