Literature summary for 2.4.1.270 extracted from

Fernandes, C.; Mendes, V.; Costa, J.; Empadinhas, N.; Jorge, C.; Lamosa, P.; Santos, H.; da Costa, M.S.
Two alternative pathways for the synthesis of the rare compatible solute mannosylglucosylglycerate in Petrotoga mobilis (2010), J. Bacteriol., 192, 1624-1633.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Petrotoga mobilis

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	in the presence of 0.1, 2.0, or 5.0 mM EDTA, the enzyme retaines 85, 30, and 23% of its maximal activity, respectively	Petrotoga mobilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate	pH 8.5, 60°C	Petrotoga mobilis
1	-	GDP-mannose	pH 8.5, 60°C	Petrotoga mobilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency	Petrotoga mobilis
Co2+	not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency	Petrotoga mobilis
Mg2+	not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency	Petrotoga mobilis
Mn2+	not strictly dependent on divalent cations, but the presence of 1 mM Mn2+, Ca2+, Mg2+, or Co2+ stimulates its activity in this order of efficiency	Petrotoga mobilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	gel filtration	Petrotoga mobilis
59000	-	1 * 59000, SDS-PAGE	Petrotoga mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate	Petrotoga mobilis	the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the rare solute mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation	GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate	-	?

Organism

Organism	UniProt	Comment	Textmining
Petrotoga mobilis	A9BHJ0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Petrotoga mobilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate	the enzyme is involved in the phosphorylating pathway for synthesis of the solute mannosylglucosylglycerate. In Petrotoga mobilis two alternative pathways for the synthesis of the rare solute mannosylglucosylglycerate are proposed. The first one is a a phosphorylating pathway (with a phosphorylated intermediate) from 3-phosphoglycerate and UDP-glucose to the final solute. The second nonphosphorylating pathway (no phosphorylated intermediates) could represent an alternative route for the synthesis of mannosylglucosylglycerate in Petrotoga mobilis that could lead to the direct conversion of glucosylglycerate and GDP-mannose to mannosylglucosylglycerate. Pathway multiplicity likely reflects a crucial role for mannosylglucosylglycerate in the physiology of Petrotoga mobilis mobilis during stress adaptation	Petrotoga mobilis	GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate	-	?
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate	no activity with UDP-mannose or mannose-1-phosphate as sugar donors, and 3-phospho-D-glycerate, 2-alpha-D-glucosylglycerate, and glucosyl-3-phosphoglycerate as the sugar acceptors	Petrotoga mobilis	GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 59000, SDS-PAGE	Petrotoga mobilis

Synonyms

Synonyms	Comment	Organism
MggA	-	Petrotoga mobilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Petrotoga mobilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
60	95	60°C: about 50% of maximal activity, 95°C: about 70% of maximal activity	Petrotoga mobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	half-life: 99 min	Petrotoga mobilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Petrotoga mobilis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	pH 6.0: about 50% of maximal activity, pH 10.0: about 75% of maximal activity	Petrotoga mobilis

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Release 2023.1 (January 2023)