Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cutscum | maximal activity in a detergent mixture, Triton X-100/Cutscum (1:2, v/v), 0.1 mg/ml | Homo sapiens | |
Triton X-100 | maximal activity in a detergent mixture, Triton X-100/Cutscum (1:2, v/v), 0.1 mg/ml | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
UDP-galactose | pH 6.8, 37°C | Homo sapiens | |
0.003 | - |
beta-D-glucosyl-(1<->1)-ceramide | pH 6.8, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme is inactive without added metal ions. Maximal activity is obtained in the presence of 1.0 mM Mn2+/Mg2+ (1:1) | Homo sapiens | |
Mn2+ | optimum activity is obtained in the presence of 1.0 mM Mn2+/Mg2+ (1:1) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide | Homo sapiens | the enzyme is involved in the synthesis of several different major classes of glycosphingolipids | UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
renal proximal tubule | from normal and familial hypercholesterolemic homozygotes. Urinary proximal tubular cells from familial hypercholesterolemic homozygous patients contain glucosylceramide beta-1,4-galactosyltransferase activity 35% higher as compared to control cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide | - |
Homo sapiens | UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? | |
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide | the enzyme is involved in the synthesis of several different major classes of glycosphingolipids | Homo sapiens | UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDPgalactose:glucosylceramide beta1->4-galactosyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | elevated glucosylceramide beta-1,4-galactosyltransferase activity may contribute to the storage of lactosylceramide urinary proximal tubular cells from familial hypercholesterolemic patients | Homo sapiens |