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Literature summary for 2.4.1.274 extracted from
- Purification, cDNA cloning, and expression of UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase from rat brain (1998), J. Biol. Chem., 273, 13570-13577.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sf9 cells | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0237 | - |
beta-D-glucosyl-(1<->1)-ceramide | pH 7.4, 37°C | Rattus norvegicus |  |
| 0.0806 | - |
UDP-galactose | pH 7.4, 37°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+ | Rattus norvegicus | |
| Mg2+ | the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+ | Rattus norvegicus | |
| Mn2+ | the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44776 | - |
x * 44776, calculated from sequence | Rattus norvegicus |
| 61000 | - |
x * 61000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide | Rattus norvegicus | - |
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains about 15% N-linked carbohydrate. The polypeptide contains eight putative glycosylation sites | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | high activity | Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | low activity | Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| spleen | low activity | Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide | - |
Rattus norvegicus | UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Rattus norvegicus |
| ? | x * 44776, calculated from sequence | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LacCer synthase | - |
Rattus norvegicus |
| lactosylceramide synthase | - |
Rattus norvegicus |
| UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
cacodylate buffer | Rattus norvegicus |
| 7.4 | - |
assay at | Rattus norvegicus |
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