Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.275 extracted from

  • Basu, M.; Weng, S.A.; Tang, H.; Khan, F.; Rossi, F.; Basu, S.
    Biosynthesis in vitro of neolactotetraosylceramide by a galactosyltransferase from mouse T-lymphoma: purification and kinetic studies; synthesis of neolacto and polylactosamine core (1996), Glycoconj. J., 13, 423-432.
    View publication on PubMed
Search for more literature for 2.4.1.275

General Stability

General Stability Organism
purified enzyme is partially stabilized in the presence of phospholipid liposomes Mus musculus
the purified GalT-4 is very unstable and loses its enzymatic activity within 48 h. The enzymatic activity could be partially stabilized in the presence of N-acetylglucosamine and Mn2+, at least for 2 weeks at 4°C Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA completely inhibited by addition of EDTA Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 N-acetyl-beta-D-galactosaminopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->)-ceramide pH 7.2, 37°C Mus musculus
0.25
-
 UDP-galactose pH 7.2, 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus
Fe3+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus
Mg2+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus
Mn2+ Mn2+ is essential for mouse lymphoma GalT-4 but could be partially replaced by Mg2+, Ca2+, or Fe3+ Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
P-1798 cell mineral oil induced Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.344
-
 pH 7.2, 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->)-ceramide
-
 Mus musculus ?
-
 ?
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide absolute requirement for an N-acetylglucosamine residue in the substrate since the lyso-derivative of the acceptor glycolipid is completely inactive as substrate Mus musculus UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?

Synonyms

Synonyms Comment Organism
GalT-4
-
 Mus musculus
UDP-Gal:GLcNAcbeta1-3Galbeta1-4Glc-Cer beta1-4galactosyltransferase
-
 Mus musculus
UDP-Gal:Lc3 beta1-4galactosyltransferase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Mus musculus