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Literature summary for 2.4.1.278 extracted from

  • Lee, H.Y.; Chung, H.S.; Hang, C.; Khosla, C.; Walsh, C.T.; Kahne, D.; Walker, S.
    Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway (2004), J. Am. Chem. Soc., 126, 9924-9925.
    View publication on PubMed
Search for more literature for 2.4.1.278

Application

Application Comment Organism
medicine potential use to make unnatural macrolides for use as antibacterial Saccharopolyspora erythraea

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of EryCIII with the GroEL/ES chaperone complex greatly enhances the expression of soluble EryCIII protein Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharopolyspora erythraea

Storage Stability

Storage Stability Organism
4°C, activity of concentrated enzyme stored overnight is more than 3 times lower than that of freshly purified enzyme. Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. TDP-D-desosamine Saccharopolyspora erythraea dTDP + erythromycin D product detected by HPLC. Identity is conformed via mass spectrometry and chromatographic comparison with an authentic reference standard ?
additional information no activity with: TDP-D-mycaminose, 6-deoxy-erythronolide B, 10-deoxymethynolide Saccharopolyspora erythraea ?
-
 ?

Synonyms

Synonyms Comment Organism
EryCIII
-
 Saccharopolyspora erythraea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharopolyspora erythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 3-alpha-mycarosylerythronolide B pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase Saccharopolyspora erythraea
1.7
-
 dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase Saccharopolyspora erythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharopolyspora erythraea