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Literature summary for 2.4.1.284 extracted from

  • Park, J.; Park, S.; Nepal, K.; Han, A.; Ban, Y.; Yoo, Y.; Kim, E.; Kim, E.; Kim, D.; Sohng, J.; Yoon, Y.
    Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation (2011), Nat. Chem. Biol., 7, 843-852.
    View publication on PubMed
Search for more literature for 2.4.1.284

Cloned(Commentary)

Cloned (Comment) Organism
KanF cannot be functionally expressed in Escherichia coli Streptomyces kanamyceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 2-deoxystreptamine using UDP-D-glucose as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus
0.32
-
 2-deoxystreptamine using UDP-N-acetyl-alpha-D-glucosamine as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus
0.44
-
 UDP-D-glucose in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl22, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus
2.54
-
 UDP-N-acetyl-alpha-D-glucosamine in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus

Organism

Organism UniProt Comment Textmining
Streptomyces kanamyceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucose + 2-deoxystreptamine best substrate Streptomyces kanamyceticus UDP + 2'-deamino-2'-hydroxyparomamine
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine reaction of 2.4.1.283, one fifth of the catalytic efficiency with UDP-D-glucose Streptomyces kanamyceticus UDP + 2'-N-acetylparomamine
-
 ?

Synonyms

Synonyms Comment Organism
kanF
-
 Streptomyces kanamyceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 UDP-D-glucose in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus
0.33
-
 UDP-N-acetyl-alpha-D-glucosamine in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
 UDP-N-acetyl-alpha-D-glucosamine in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus
0.66
-
 UDP-D-glucose in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C Streptomyces kanamyceticus