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Literature summary for 2.4.1.289 extracted from

  • Grzegorzewicz, A.E.; Ma, Y.; Jones, V.; Crick, D.; Liav, A.; McNeil, M.R.
    Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL (2008), Microbiology, 154, 3724-3730.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates the enzyme activity Mycobacterium tuberculosis

Application

Application Comment Organism
drug development WbbL is a good target for the development of antituberculosis therapeutics Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
gene wbb, overexpression in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Mycobacterium tuberculosis 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information WbbL shows an absolute requirement for divalent cations Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol Mycobacterium tuberculosis natural acceptor substrate GlcNAc-P-P-decaprenyl dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol Mycobacterium tuberculosis H37Rv natural acceptor substrate GlcNAc-P-P-decaprenyl dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
gene wbbL
-
Mycobacterium tuberculosis H37Rv
-
gene wbbL
-

Reaction

Reaction Comment Organism Reaction ID
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol kinetic analysis shows that WbbL uses a ternary complex mechanism reaction Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
Mycobacterium tuberculosis dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol natural acceptor substrate GlcNAc-P-P-decaprenyl Mycobacterium tuberculosis dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
Mycobacterium tuberculosis H37Rv dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol natural acceptor substrate GlcNAc-P-P-decaprenyl Mycobacterium tuberculosis H37Rv dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?

Synonyms

Synonyms Comment Organism
cell wall rhamnosyltransferase
-
Mycobacterium tuberculosis
WbbL
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
assay at Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function WbbL catalyzes the transfer of an alpha-L-rhamnopyranose residue from dTDP-Rha to decaprenyldiphosphoryl-alpha-D-N-acetyl glucosamine, GlcNAc-P-PDP, to form alpha-L-Rhap-(1->3)-alpha-D-GlcNAc-P-P-DP, which is then further elongated with Galf and Araf units and finally mycolylated and attached to the peptidoglycan. The enzyme is essential for Mycobacterium tuberculosis viability and is absent in eukaryotic cells Mycobacterium tuberculosis