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Literature summary for 2.4.1.290 extracted from
- In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system (2006), Biochemistry, 45, 13659-13669.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pglA, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Campylobacter jejuni | starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline | ? | - |
? |  |
| additional information | Campylobacter jejuni NCTC 11168 | starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni | one N-acetylgalactosamine is linked to the polyisoprenepyrophosphate-bound N,N'-diacetylbacillosamine by Cj1125c (PglA) to form GalNAc-alpha1,3-Bac2,4diNAc-alpha1-PP-Und | UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni | - |
gene Cj1125c or pglA | - |
| Campylobacter jejuni NCTC 11168 | - |
gene Cj1125c or pglA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged PglA from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography | Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline | Campylobacter jejuni | ? | - |
? | |
| additional information | starting from UDP-GalNAc, UDP-Glc, Und-P and chemically synthesized UDP-Bac-2,4-NAc, the reactions of recombinant PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel to synthesize the bacterial glycan heptasaccharide, overview. Presence of GST-PglF, PglE and PglD in the same reaction vessel does not inhibit the downstream reactions. In N-linked glycoproteins the heptasaccharide is covalently attached at the N,N'-diacetylbacillosamine through a beta-linkage to the amide nitrogen of an asparagine side chain in the canonical sequence Asn-Xaa-Ser/Thr motif, where Xaa can be any amino acid except proline | Campylobacter jejuni NCTC 11168 | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni | UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | one N-acetylgalactosamine is linked to the polyisoprenepyrophosphate-bound N,N'-diacetylbacillosamine by Cj1125c (PglA) to form GalNAc-alpha1,3-Bac2,4diNAc-alpha1-PP-Und | Campylobacter jejuni | UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cj1125c | - |
Campylobacter jejuni |
| PglA | - |
Campylobacter jejuni |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the biosynthesis of the sugar 2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose, termed N,N'-diacetylbacillosamine or Bac2,4diNAc, the first carbohydrate in the glycoprotein N-linked heptasaccharide, in vitro biosynthesis of the complete heptasaccharide lipid-linked donor by coupling the action of eight enzymes, PglF, PglE, PglD, PglC, PglA, PglJ, PglH, and PglI, in the Pgl pathway | Campylobacter jejuni |
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