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Literature summary for 2.4.1.292 extracted from

  • Glover, K.J.; Weerapana, E.; Imperiali, B.
    In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation (2005), Proc. Natl. Acad. Sci. USA, 102, 14255-14259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pglH, overexpression of His-tagged PglH in Escherichia coli Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane asociated Campylobacter jejuni 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, recombinant His-tagged PglH, SDS-PAGE Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol Campylobacter jejuni
-
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
gene pglH, pgl gene cluster
-
Campylobacter jejuni NCTC 11168
-
gene pglH, pgl gene cluster
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PglH from Escherichia coli by nickel affinity chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
Campylobacter jejuni 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
?
additional information in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH Campylobacter jejuni ?
-
?
additional information in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH Campylobacter jejuni NCTC 11168 ?
-
?

Subunits

Subunits Comment Organism
? x * 41000, recombinant His-tagged PglH, SDS-PAGE Campylobacter jejuni

Synonyms

Synonyms Comment Organism
PglH
-
Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Campylobacter jejuni

General Information

General Information Comment Organism
metabolism PglH is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenylpyrophosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the Und-PP-linked heptasaccharide. Elongation of the trisaccharide with PglH results in a hexasaccharide revealing the polymerase activity of PglH Campylobacter jejuni