Cloned (Comment) | Organism |
---|---|
expression in Streptomyces venezuelae. Reconstructed the kanamycin pathway stepwise in a mutant strain of Streptomyces venezuelae lacking the genes for biosynthesis of the native deoxysugar by transplanting plasmids harboring different combinations of putative genes from Streptomyces kanamyceticus in Streptomyces venezuelae and then carrying out biochemical analysis and chemical characterization | Streptomyces kanamyceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
UDP-alpha-D-kanosamine | 37°C, pH not specified in the publication, cosubstrate: paromamine | Streptomyces kanamyceticus | |
0.05 | - |
paromamine | 37°C, pH not specified in the publication, cosubstrate: UDP-alpha-D-kanosamine | Streptomyces kanamyceticus | |
0.25 | - |
paromamine | 37°C, pH not specified in the publication, cosubstrate: UDP-alpha-D-glucose | Streptomyces kanamyceticus | |
0.26 | - |
UDP-alpha-D-glucose | 37°C, pH not specified in the publication, cosubstrate: paromamine | Streptomyces kanamyceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine | Streptomyces kanamyceticus | the enzyme is involved in the biosynthetic pathway of kanamycins | UDP + kanamycin A | - |
? | |
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine | Streptomyces kanamyceticus | the enzyme is involved in the biosynthetic pathway of kanamycins | UDP + kanamycin X | - |
? | |
UDP-alpha-D-kanosamine + neamine | Streptomyces kanamyceticus | the enzyme is involved in the biosynthetic pathway of kanamycins | UDP + kanamycin B | - |
? | |
UDP-alpha-D-kanosamine + paromamine | Streptomyces kanamyceticus | the enzyme is involved in the biosynthetic pathway of kanamycins | UDP + kanamycin C | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces kanamyceticus | Q65CC7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme can also accept UDP-alpha-D-glucose with lower efficiency | Streptomyces kanamyceticus | ? | - |
? | |
UDP-alpha-D-glucose + paromamine | paromamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside. UDP-alpha-D-kanosamine is a better glycosyl donor than UDP-alpha-D-glucose | Streptomyces kanamyceticus | UDP + ? | - |
? | |
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine | the enzyme is involved in the biosynthetic pathway of kanamycins | Streptomyces kanamyceticus | UDP + kanamycin A | - |
? | |
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine | UDP-alpha-D-kanosamine i.e. uridine 5'-[3-(3-amino-3-deoxy-alpha-D-glucopyranosyl) diphosphate] | Streptomyces kanamyceticus | UDP + kanamycin A | kanamycin A i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside | ? | |
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine | - |
Streptomyces kanamyceticus | UDP + kanamycin X | kanamycin X i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside | ? | |
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine | the enzyme is involved in the biosynthetic pathway of kanamycins | Streptomyces kanamyceticus | UDP + kanamycin X | - |
? | |
UDP-alpha-D-kanosamine + neamine | the enzyme is involved in the biosynthetic pathway of kanamycins | Streptomyces kanamyceticus | UDP + kanamycin B | - |
? | |
UDP-alpha-D-kanosamine + neamine | neamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside | Streptomyces kanamyceticus | UDP + kanamycin B | kanamycin B i.e. (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside | ? | |
UDP-alpha-D-kanosamine + paromamine | the enzyme is involved in the biosynthetic pathway of kanamycins | Streptomyces kanamyceticus | UDP + kanamycin C | - |
? | |
UDP-alpha-D-kanosamine + paromamine | paromamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside | Streptomyces kanamyceticus | UDP + kanamycin C | kanamycin C i.e. (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside | ? |
Synonyms | Comment | Organism |
---|---|---|
KanE | - |
Streptomyces kanamyceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
UDP-alpha-D-glucose | 37°C, pH not specified in the publication | Streptomyces kanamyceticus | |
0.056 | - |
UDP-alpha-D-kanosamine | 37°C, pH not specified in the publication | Streptomyces kanamyceticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
UDP-alpha-D-glucose | 37°C, pH not specified in the publication, cosubstrate: paromamine | Streptomyces kanamyceticus | |
1.87 | - |
UDP-alpha-D-kanosamine | 37°C, pH not specified in the publication, cosubstrate: paromamine | Streptomyces kanamyceticus |