Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a C-terminal His8 fusion protein | Streptomyces niveus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTDP-beta-L-rhamnose | - |
Streptomyces niveus | |
novobiocic acid | above 0.06 mM | Streptomyces niveus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
dTDP-beta-L-noviose | pH 6.0, 22°C | Streptomyces niveus | |
0.032 | - |
novobiocic acid | pH 6.0, 22°C | Streptomyces niveus | |
0.039 | - |
cyclonovobiocic acid | pH 6.0, 22°C | Streptomyces niveus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Streptomyces niveus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-noviose + novobiocic acid | Streptomyces niveus | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin | dTDP + demethyldecarbamoyl novobiocin | i.e. demethyldecarbamoyl novobiocin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces niveus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
C-terminal His8 fusion protein | Streptomyces niveus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-noviose + novobiocic acid | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin | Streptomyces niveus | dTDP + demethyldecarbamoyl novobiocin | i.e. demethyldecarbamoyl novobiocin | ? | |
dTDP-beta-L-noviose + novobiocic acid | no activity with TDP-L-rhamnose | Streptomyces niveus | dTDP + demethyldecarbamoyl novobiocin | - |
? | |
dTDP-L-noviose + 4-nitrophenol | kcat/Km is 208000fold lower compared to the value for the natural substrate novobiocic acid | Streptomyces niveus | dTDP + ? | - |
? | |
dTDP-L-noviose + 7-hydroxy-4-methylcoumarin | kcat/Km is 142000fold lower compared to the value for the natural substrate novobiocic acid | Streptomyces niveus | dTDP + ? | - |
? | |
dTDP-L-noviose + 7-hydroxycoumarin | kcat/Km is 172000fold lower compared to the value for the natural substrate novobiocic acid | Streptomyces niveus | dTDP + ? | - |
? | |
dTDP-L-noviose + cyclonovobiocic acid | kcat/KM is 30% compared to the value for the natural substrate novobiocic acid | Streptomyces niveus | dTDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Streptomyces niveus |
Synonyms | Comment | Organism |
---|---|---|
NovM | - |
Streptomyces niveus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Streptomyces niveus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
118 | - |
cyclonovobiocic acid | pH 6.0, 22°C | Streptomyces niveus | |
226 | - |
dTDP-beta-L-noviose | pH 6.0, 22°C | Streptomyces niveus | |
313 | - |
novobiocic acid | pH 6.0, 22°C | Streptomyces niveus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Streptomyces niveus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0835 | - |
dTDP-beta-L-rhamnose | pH 6.0, 22°C | Streptomyces niveus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin | Streptomyces niveus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
4-nitrophenol | pH 6.0, 22°C | Streptomyces niveus | |
0.057 | - |
7-hydroxycoumarin | pH 6.0, 22°C | Streptomyces niveus | |
0.069 | - |
7-hydroxy-4-methylcoumarin | pH 6.0, 22°C | Streptomyces niveus | |
3026 | - |
cyclonovobiocic acid | pH 6.0, 22°C | Streptomyces niveus | |
9781 | - |
novobiocic acid | pH 6.0, 22°C | Streptomyces niveus |