Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | detergents in the assay do not increase glycosyl transfer | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed as a GST-fusion protein encoded by pCM227 in DH5alpha | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHAPS | activity is diminished in the presence of CHAPS at concentrations of 0.1250.6% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside | pH 7.0, 37°C | Escherichia coli | |
1.2 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, yields 38% activity, respectively, compared with 5 mM MnCl2 | Escherichia coli | |
Mg2+ | 5 mM, yields 11% activity, respectively, compared with 5 mM MnCl2 | Escherichia coli | |
Mn2+ | optimal activity in presence of 2.5 mM Mn2+. Without addition of divalent metal the activity is less than 4.5% of the activity in presence of Mn2+ | Escherichia coli | |
Zn2+ | 5 mM, yields 9% activity, respectively, compared with 5 mM MnCl2 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli VW187 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside | synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol | Escherichia coli | UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside | - |
? | |
UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside | synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol | Escherichia coli VW187 | UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-Gal:GlcNAcalpha-pyrophosphate-R beta1,3-galactosyltransferase | - |
Escherichia coli |
WbbD | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | broad optimum | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a mutant strain with a defective wbbD gene is unable to form O7 LPS and lacks this specific galactosyltransferase activity. The normal phenotype is restored by complementing the mutant with the cloned wbbD gene | Escherichia coli |
physiological function | the enzyme is involved in the biosynthesis of the O7-specific lipopolysaccharide | Escherichia coli |