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Literature summary for 2.4.1.306 extracted from
- The wbnH gene of Escherichia coli O86:H2 encodes an alpha-1,3-N-acetylgalactosaminyl transferase involved in the O-repeating unit biosynthesis (2006), Biochem. Biophys. Res. Commun., 344, 631-639.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent metal cations are not required for enzyme catalysis | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Escherichia coli |
| 38148 | - |
x * 38148, calculated from sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol | Escherichia coli | the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol | - |
? |  |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol | Escherichia coli 86:H2 | the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli 86:H2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the natural substrate N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol is difficult to isolate from natural bacterial sources due to the low abundance. The highly hydrophobic feature of the 55-carbon undecaprenyl chain makes it hard to handle | Escherichia coli | ? | - |
? | |
| additional information | the natural substrate N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol is difficult to isolate from natural bacterial sources due to the low abundance. The highly hydrophobic feature of the 55-carbon undecaprenyl chain makes it hard to handle | Escherichia coli 86:H2 | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3 | 95% of the activity with UDP-N-acetyl-D-galactosamine + D-GalNAc-alpha-diphospho-11-phenoxyundecanol | Escherichia coli | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3 | 95% of the activity with UDP-N-acetyl-D-galactosamine + D-GalNAc-alpha-diphospho-11-phenoxyundecanol | Escherichia coli 86:H2 | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol | synthetic acceptor substrate. Diphosphate and hydrophobic lipid moiety are structural requirements for WbnH activity | Escherichia coli | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol | synthetic acceptor substrate. Diphosphate and hydrophobic lipid moiety are structural requirements for WbnH activity | Escherichia coli 86:H2 | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria | Escherichia coli | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria | Escherichia coli 86:H2 | UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Escherichia coli |
| ? | x * 38148, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| WbnH | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at, | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | - |
- |
6.4 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria | Escherichia coli |
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