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Literature summary for 2.4.1.308 extracted from

  • Yi, W.; Shao, J.; Zhu, L.; Li, M.; Singh, M.; Lu, Y.; Lin, S.; Li, H.; Ryu, K.; Shen, J.; Guo, H.; Yao, Q.; Bush, C.A.; Wang, P.G.
    Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide (2005), J. Am. Chem. Soc., 127, 2040-2041.
    View publication on PubMed
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Application

Application Comment Organism
synthesis stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing us an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-tagged fusion protein Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q58YV9
-
-
Escherichia coli O86 Q58YV9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 Escherichia coli GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 Escherichia coli O86 GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
GDP-beta-L-fucose + beta-D-Gal-OMe 15% of the activity compared to beta-Gal-(1->3)-alpha-GalNAc-OMe Escherichia coli ?
-
 ?
GDP-beta-L-fucose + beta-D-Gal-OMe 15% of the activity compared to beta-Gal-(1->3)-alpha-GalNAc-OMe Escherichia coli O86 ?
-
 ?
additional information WbnK requires a terminal beta-1,3-linked Gal sugar sequence as acceptor Escherichia coli ?
-
 ?
additional information WbnK requires a terminal beta-1,3-linked Gal sugar sequence as acceptor Escherichia coli O86 ?
-
 ?

Synonyms

Synonyms Comment Organism
WbnK
-
 Escherichia coli