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Literature summary for 2.4.1.310 extracted from

  • Nakayama, A.; Okano, A.; Feng, Y.; Collins, J.C.; Collins, K.C.; Walsh, C.T.; Boger, D.L.
    Enzymatic glycosylation of vancomycin aglycon: completion of a total synthesis of vancomycin and N- and C-terminus substituent effects of the aglycon substrate (2014), Org. Lett., 16, 3572-3575.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.310

Application

Application Comment Organism
synthesis in vitro sequential glycosylations of the vancomycin aglycon catalyzed by the glycosyltransferases GtfE and GtfD and total synthesis of vancomycin and derivatives. For GtfE, presence of reductant tris(2-carboxyethyl)phosphine (TCEP, 2 mM) is crucial Amycolatopsis orientalis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Amycolatopsis orientalis

Inhibitors

Inhibitors Comment Organism Structure
CHAPS
-
 Amycolatopsis orientalis
Triton X-100
-
 Amycolatopsis orientalis
Tween 20
-
 Amycolatopsis orientalis

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO up to 10% v/v, tolerated Amycolatopsis orientalis

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis
-
-
-
Amycolatopsis orientalis ATCC 19795
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information study on reaction after modification of the vancomycin aglycon. Modification at the N-terminus amine of vancomycin aglycon is well tolerated, whereas C-terminus modification with removal of the acidic carboxylic acid results in a slower, less effective initial glycosylation Amycolatopsis orientalis ?
-
 ?
additional information study on reaction after modification of the vancomycin aglycon. Modification at the N-terminus amine of vancomycin aglycon is well tolerated, whereas C-terminus modification with removal of the acidic carboxylic acid results in a slower, less effective initial glycosylation Amycolatopsis orientalis ATCC 19795 ?
-
 ?
UDP-alpha-D-glucose + vancomycin aglycone
-
 Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
 ?
UDP-alpha-D-glucose + vancomycin aglycone
-
 Amycolatopsis orientalis ATCC 19795 UDP + devancoaminyl vancomycin
-
 ?

Synonyms

Synonyms Comment Organism
GtfE
-
 Amycolatopsis orientalis