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Literature summary for 2.4.1.315 extracted from

  • Andres, E.; Martinez, N.; Planas, A.
    Expression and characterization of a Mycoplasma genitalium glycosyltransferase in membrane glycolipid biosynthesis: Potential target against Mycoplasma infections (2011), J. Biol. Chem., 286, 35367-35379.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.315

Activating Compound

Activating Compound Comment Organism Structure
dioleoylphosphatidylglycerol kcat linearly increases with dioleoylphosphatidylglycerol concentration Mycoplasma genitalium

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycoplasma genitalium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 UDP-alpha-D-glucose above 0.05, acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
0.087
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
0.09
-
 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
0.234
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
0.27
-
 1,2-diacyl-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
0.479
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium

Organism

Organism UniProt Comment Textmining
Mycoplasma genitalium Q9ZB73 ATCC 33530
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme transfers glucosyl and galactosyl residues from UDP-Glc and UDP-Gal to dioleoylglycerol acceptor to form the corresponding beta-glycosyl-dioleoylglycerol, which then acts as acceptor to give beta-diglycosyl-dioleoylglycerol products. Enzyme sequentially adds glycosyl residues with beta-1,6 connectivity, the first glycosyltransferase activity to dioleoylglycerol being about 1 order of magnitude higher than the second to monoglucosyldioleoylglycerol Mycoplasma genitalium ?
-
 ?
UDP-alpha-D-galactose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 9% of the specific activity with 1,2-diacyl-sn-glycerol Mycoplasma genitalium 1,2-diacyl-3-O-[alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 2.2% of the specific activity with 1,2-diacyl-sn-glycerol Mycoplasma genitalium 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
 ?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
-
 Mycoplasma genitalium 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP main product ?
UDP-alpha-D-glucose + 1,2-diacyl-sn-glycerol
-
 Mycoplasma genitalium 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol + UDP
-
 ?

Synonyms

Synonyms Comment Organism
MG517
-
 Mycoplasma genitalium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
0.023
-
 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
0.03
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
0.27
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
1.1
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
1.13
-
 1,2-diacyl-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
0.063
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
0.08
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
3.1
-
 UDP-alpha-D-glucose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium
4
-
 1,2-diacyl-sn-glycerol donor UDP-alpha-D-galactose, pH 8.0, 28°C Mycoplasma genitalium
5
-
 UDP-alpha-D-galactose acceptor 1,2-diacyl-sn-glycerol, pH 8.0, 28°C Mycoplasma genitalium