Literature summary for 2.4.1.321 extracted from

Hildebrand, A.; Szewczyk, E.1, Lin, H.; Kasuga, T.; Fan, Z.
Engineering Neurospora crassa for improved cellobiose and cellobionate production (2015), Appl. Environ. Microbiol., 81, 597-603.

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Application

Application	Comment	Organism
synthesis	an engineered strain of Neurospora crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out produces cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. Further modification by knock-out of catabolite repression genes, cre-1 and ace-1, leads to improved cellobiose dehydrogenase and exoglucanase expression but not to an improvement in cellobiose or cellobionate production. Deletion of the cellobionate phosphorylase gene NdvB from the genome of F5 lacking ace-1 and cre-1 to prevent the consumption of cellobiose and cellobionate results in a slightly reduced hydrolysis rate and in convertsion of 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 lackong only ace-1 cre-1	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	Q7S0S2	-	-
Neurospora crassa DSM 1257	Q7S0S2	-	-

Synonyms

Synonyms	Comment	Organism
NdvB	-	Neurospora crassa

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Release 2023.1 (January 2023)