Crystallization (Comment) | Organism |
---|---|
structures of ligand-free and complex forms with cellobionic acid, gluconic acid, and a synthetic disaccharide product from glucuronic acid, up to 1.6 A resolution. The active site is located near the dimer interface. At subsite +1, the carboxylate group of gluconic acid and cellobionic acid is recognized by residues Arg609 and Lys613. Additionally, one residue from the neighboring protomer (Gln190) is involved. These residues are critical for the binding and catalysis of gluconic acid and glucuronic acid | Saccharophagus degradans |
Protein Variants | Comment | Organism |
---|---|---|
Q190A | about 10% of wild-type activity | Saccharophagus degradans |
R609A | complete loss of activity | Saccharophagus degradans |
R613A | complete loss of activity | Saccharophagus degradans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
D-gluconate | wild-type, pH 6.5, 30°C | Saccharophagus degradans | |
13.4 | - |
D-glucuronate | wild-type, pH 6.5, 30°C | Saccharophagus degradans | |
41 | - |
D-gluconate | mutant Q190A, pH 6.5, 30°C | Saccharophagus degradans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88700 | - |
2 * 88700, SDS-PAGE | Saccharophagus degradans |
147000 | - |
gel filtration | Saccharophagus degradans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharophagus degradans | Q21MB1 | - |
- |
Saccharophagus degradans DSM 17024 | Q21MB1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9 | - |
substrate 4-O-beta-D-glucopyranosyl-D-gluconate, mutant Q190A, pH 6.5, 30°C | Saccharophagus degradans |
62 | - |
substrate 4-O-beta-D-glucopyranosyl-D-gluconate, wild-type, pH 6.5, 30°C | Saccharophagus degradans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
Saccharophagus degradans | alpha-D-glucose 1 phosphate + D-gluconate | - |
r | |
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
Saccharophagus degradans DSM 17024 | alpha-D-glucose 1 phosphate + D-gluconate | - |
r | |
alpha-D-glucose 1 phosphate + D-gluconate | - |
Saccharophagus degradans | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1 phosphate + D-gluconate | - |
Saccharophagus degradans DSM 17024 | 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate | - |
r | |
alpha-D-glucose 1 phosphate + D-glucuronate | - |
Saccharophagus degradans | 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate | - |
r | |
alpha-D-glucose 1 phosphate + D-glucuronate | - |
Saccharophagus degradans DSM 17024 | 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate | - |
r | |
additional information | sequential bi-bi mechanism | Saccharophagus degradans | ? | - |
? | |
additional information | sequential bi-bi mechanism | Saccharophagus degradans DSM 17024 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 88700, SDS-PAGE | Saccharophagus degradans |
Synonyms | Comment | Organism |
---|---|---|
CBAP | - |
Saccharophagus degradans |
cep94B | - |
Saccharophagus degradans |
Sde_0906 | - |
Saccharophagus degradans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
D-gluconate | mutant Q190A, pH 6.5, 30°C | Saccharophagus degradans | |
34.6 | - |
D-glucuronate | wild-type, pH 6.5, 30°C | Saccharophagus degradans | |
130 | - |
D-gluconate | wild-type, pH 6.5, 30°C | Saccharophagus degradans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
D-gluconate | mutant Q190A, pH 6.5, 30°C | Saccharophagus degradans | |
2.58 | - |
D-glucuronate | wild-type, pH 6.5, 30°C | Saccharophagus degradans | |
76 | - |
D-gluconate | wild-type, pH 6.5, 30°C | Saccharophagus degradans |