Literature summary for 2.4.1.321 extracted from

Nam, Y.W.; Nihira, T.; Arakawa, T.; Saito, Y.; Kitaoka, M.; Nakai, H.; Fushinobu, S.
Crystal structure and substrate recognition of cellobionic ccid phosphorylase, which plays a key role in oxidative cellulose degradation by microbes (2015), J. Biol. Chem., 290, 18281-18292.

Search for more literature for 2.4.1.321

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of ligand-free and complex forms with cellobionic acid, gluconic acid, and a synthetic disaccharide product from glucuronic acid, up to 1.6 A resolution. The active site is located near the dimer interface. At subsite +1, the carboxylate group of gluconic acid and cellobionic acid is recognized by residues Arg609 and Lys613. Additionally, one residue from the neighboring protomer (Gln190) is involved. These residues are critical for the binding and catalysis of gluconic acid and glucuronic acid	Saccharophagus degradans

Crystallization (Comment)

Organism

structures of ligand-free and complex forms with cellobionic acid, gluconic acid, and a synthetic disaccharide product from glucuronic acid, up to 1.6 A resolution. The active site is located near the dimer interface. At subsite +1, the carboxylate group of gluconic acid and cellobionic acid is recognized by residues Arg609 and Lys613. Additionally, one residue from the neighboring protomer (Gln190) is involved. These residues are critical for the binding and catalysis of gluconic acid and glucuronic acid

Saccharophagus degradans

Protein Variants

Protein Variants	Comment	Organism
Q190A	about 10% of wild-type activity	Saccharophagus degradans
R609A	complete loss of activity	Saccharophagus degradans
R613A	complete loss of activity	Saccharophagus degradans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
1.7	-	D-gluconate	wild-type, pH 6.5, 30°C	Saccharophagus degradans
13.4	-	D-glucuronate	wild-type, pH 6.5, 30°C	Saccharophagus degradans
41	-	D-gluconate	mutant Q190A, pH 6.5, 30°C	Saccharophagus degradans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88700	-	2 * 88700, SDS-PAGE	Saccharophagus degradans
147000	-	gel filtration	Saccharophagus degradans

Organism

Organism	UniProt	Comment	Textmining
Saccharophagus degradans	Q21MB1	-	-
Saccharophagus degradans DSM 17024	Q21MB1	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9	-	substrate 4-O-beta-D-glucopyranosyl-D-gluconate, mutant Q190A, pH 6.5, 30°C	Saccharophagus degradans
62	-	substrate 4-O-beta-D-glucopyranosyl-D-gluconate, wild-type, pH 6.5, 30°C	Saccharophagus degradans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	Saccharophagus degradans	alpha-D-glucose 1 phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	Saccharophagus degradans DSM 17024	alpha-D-glucose 1 phosphate + D-gluconate	-	r
alpha-D-glucose 1 phosphate + D-gluconate	-	Saccharophagus degradans	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1 phosphate + D-gluconate	-	Saccharophagus degradans DSM 17024	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1 phosphate + D-glucuronate	-	Saccharophagus degradans	4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate	-	r
alpha-D-glucose 1 phosphate + D-glucuronate	-	Saccharophagus degradans DSM 17024	4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate	-	r
additional information	sequential bi-bi mechanism	Saccharophagus degradans	?	-	?
additional information	sequential bi-bi mechanism	Saccharophagus degradans DSM 17024	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 88700, SDS-PAGE	Saccharophagus degradans

Synonyms

Synonyms	Comment	Organism
CBAP	-	Saccharophagus degradans
cep94B	-	Saccharophagus degradans
Sde_0906	-	Saccharophagus degradans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
24	-	D-gluconate	mutant Q190A, pH 6.5, 30°C	Saccharophagus degradans
34.6	-	D-glucuronate	wild-type, pH 6.5, 30°C	Saccharophagus degradans
130	-	D-gluconate	wild-type, pH 6.5, 30°C	Saccharophagus degradans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.58	-	D-gluconate	mutant Q190A, pH 6.5, 30°C	Saccharophagus degradans
2.58	-	D-glucuronate	wild-type, pH 6.5, 30°C	Saccharophagus degradans
76	-	D-gluconate	wild-type, pH 6.5, 30°C	Saccharophagus degradans