KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
dTDP-beta-L-daunosamine | pH not specified in the publication, temperature not specified in the publication | Amycolatopsis orientalis | |
0.232 | - |
dTDP-beta-L-acosamine | pH not specified in the publication, temperature not specified in the publication | Amycolatopsis orientalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | Q9AFC7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-acosamine + devancosaminyl-vancomycin | - |
Amycolatopsis orientalis | dTDP + ? | - |
? | |
dTDP-beta-L-daunosamine + devancosaminyl-vancomycin | - |
Amycolatopsis orientalis | dTDP + ? | - |
? | |
dTDP-beta-L-vancosamine + devancosaminyl-vancomycin | natural substrate | Amycolatopsis orientalis | dTDP + vancomycin | - |
? | |
additional information | enzyme shows moderately relaxed donor substrate specificities for the glycosylation of its natural aglycone. It transfers 4-epi-L-vancosamine, L-daunosamine, and L-acosamine with good turnover. 2-Deoxy-L-glucose, 2-deoxy-L-rhamnose, and L-ristosamine are transferred in trace amounts, whereas for substrates containing an amino group at C-4, no product is observed | Amycolatopsis orientalis | ? | - |
? | |
UDP-4-epi-L-vancosamine + devancosaminyl-vancomycin | - |
Amycolatopsis orientalis | UDP + epi-L-vancomycin | epivancomycin is a hybrid teicoplanin/vancomycin glycopeptide | ? |
Synonyms | Comment | Organism |
---|---|---|
GtfD | - |
Amycolatopsis orientalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.13 | - |
dTDP-beta-L-daunosamine | pH not specified in the publication, temperature not specified in the publication | Amycolatopsis orientalis | |
2.25 | - |
dTDP-beta-L-acosamine | pH not specified in the publication, temperature not specified in the publication | Amycolatopsis orientalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.7 | - |
dTDP-beta-L-acosamine | pH not specified in the publication, temperature not specified in the publication | Amycolatopsis orientalis | |
56.7 | - |
dTDP-beta-L-daunosamine | pH not specified in the publication, temperature not specified in the publication | Amycolatopsis orientalis |