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Literature summary for 2.4.1.324 extracted from
- Iridoid-specific glucosyltransferase from Gardenia jasminoides (2011), J. Biol. Chem., 286, 32866-32874.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as N-terminal fusion proteins with a His6 tag in Escherichia coli strain JM109 | Gardenia jasminoides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
UDP-alpha-D-glucose | pH 8.0, 30°C | Gardenia jasminoides |  |
| 0.61 | - |
7-deoxyloganetin | pH 8.0, 30°C | Gardenia jasminoides | |
| 8.82 | - |
genipin | pH 8.0, 30°C | Gardenia jasminoides | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 7-deoxyloganetin | Gardenia jasminoides | the enzyme is involved in loganin and secologanin biosynthesis | UDP + 7-deoxyloganin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gardenia jasminoides | F8WKW5 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Gardenia jasminoides | - |
| fruit | the mRNA level gradually increases during the early stage of fruit ripening, whereas it remains low in the mature fruits | Gardenia jasminoides | - |
| leaf | - |
Gardenia jasminoides | - |
| additional information | very low expression level in the stem | Gardenia jasminoides | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 7-deoxyloganetin | the enzyme is involved in loganin and secologanin biosynthesis | Gardenia jasminoides | UDP + 7-deoxyloganin | - |
? | |
| UDP-alpha-D-glucose + 7-deoxyloganetin | does not react with 7-deoxyloganetate, weak activity toward loganetin. The enzyme exhibits no activity toward either UDP-galactose or UDP-glucuronic acid | Gardenia jasminoides | UDP + 7-deoxyloganin | i.e. 7-deoxyloganetin 1-O-beta-D-glucoside | ? | |
| UDP-alpha-D-glucose + genipin | - |
Gardenia jasminoides | UDP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDPglucose:iridoid glucosyltransferase | - |
Gardenia jasminoides |
| UGT6 | - |
Gardenia jasminoides |
| UGT85A24 | - |
Gardenia jasminoides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Gardenia jasminoides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
7-deoxyloganetin | pH 8.0, 30°C | Gardenia jasminoides | |
| 0.16 | - |
UDP-alpha-D-glucose | pH 8.0, 30°C | Gardenia jasminoides | |
| 1.04 | - |
genipin | pH 8.0, 30°C | Gardenia jasminoides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Gardenia jasminoides |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Gardenia jasminoides | mRNA level is markedly increased by adding methyl jasmonate to the cell cultures and the pattern of up-regulation correlated well with the pattern of increased enzymatic activity | up |
| Gardenia jasminoides | the mRNA level gradually increases during the early stage of fruit ripening | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
genipin | pH 8.0, 30°C | Gardenia jasminoides | |
| 0.21 | - |
7-deoxyloganetin | pH 8.0, 30°C | Gardenia jasminoides | |
| 1 | - |
UDP-alpha-D-glucose | pH 8.0, 30°C | Gardenia jasminoides | |
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