Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acholeplasma laidlawii |
recombinant overexpression in Escherichia coli strain BL21-AI membranes resulting in an upregulated phospholipid synthesis and vesiculation, acyl chain ordering of lipids during enzyme MGS production, overview | Acholeplasma laidlawii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acholeplasma laidlawii | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 1,2-diacyl-sn-glycerol | Acholeplasma laidlawii | - |
UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acholeplasma laidlawii | Q93P60 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 1,2-diacyl-sn-glycerol | - |
Acholeplasma laidlawii | UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MGS | - |
Acholeplasma laidlawii |
monoglucosyldiacylglycerol synthase | - |
Acholeplasma laidlawii |
General Information | Comment | Organism |
---|---|---|
physiological function | membrane protein monoglucosyldiacylglycerol synthase MGS is responsible for the creation of intracellular membranes when overexpressed in Escherichia coli. During 22 h of MGS induction, the lipid/protein ratio increases by 38% in MGS-expressing cells compared to control cells. Unsaturation levels remain constant for MGS cells (about 62%) but significantly decrease in control cells (from 61% to 36%). Cyclopropanated fatty acid content is lower in MGS producing cells while control cells have an increased cyclopropanation activity. Among all lipids, phosphatidylethanolamine is the most affected species in terms of cyclopropanation | Acholeplasma laidlawii |
physiological function | role of enzyme MGS protein on stress regulation and cyclopropane fatty acid synthase activity. The fatty acid composition changes in enzyme MGS-overexpressing cells, overview | Acholeplasma laidlawii |