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Literature summary for 2.4.1.34 extracted from

  • Frost, D.J.; Brandt, K.; Capobianco, J.; Goldman, R.
    Characterization of (1,3)-beta-glucan synthase in Candida albicans: microsomal assay from the yeast or mycelial morphological forms and a permeabilized whole-cell assay (1994), Microbiology, 140, 2239-2246.
No PubMed abstract available

General Stability

General Stability Organism
polyoxyethylene ether stabilizes the enzyme Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
UDPglucose
-
Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Candida albicans
-
-

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Candida albicans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0041
-
with polyoxyethylene ether in the assay but not during cell breakage Candida albicans
0.0125
-
with polyoxyethylene ether in the assay and during cell breakage Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + (1,3-beta-D-glucosyl)n
-
Candida albicans UDP + (1,3-beta-D-glucosyl)n+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Candida albicans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half life with stabilizing polyoxyethylene ether in the assay but not during cell breakage: 5.6 d Candida albicans
4
-
half life without polyoxyethylene ether in the assay but not during cell breakage: 0.92 d Candida albicans
4
-
half life without polyoxyethylene ether in the assay but during cell breakage: 5.5 d Candida albicans
4
-
half life with polyoxyethylene ether in the assay and during cell breakage: 12.1 d Candida albicans
30
-
half life with stabilizing polyoxyethylene ether in the assay but not during cell breakage: 15.9 h Candida albicans
30
-
half life without polyoxyethylene ether in the assay but not during cell breakage: 0.5 h Candida albicans
30
-
half life with stabilizing polyoxyethylene ether in the assay and during cell breakage: 18.4 h Candida albicans
30
-
half life without polyoxyethylene ether in the assay but during cell breakage: 11.8 h Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.75
-
-
Candida albicans