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Literature summary for 2.4.1.34 extracted from

  • Mulichak, A.M.; Losey, H.C.; Walsh, C.T.; Garavito, R.M.
    Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics (2001), Structure, 9, 547-557.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
two distinct N- and C-terminal halves, similar in size and topology and separated by a deep cleft, both domains contain a similar core structure of parallel beta sheets connected by alpha helices, the C-terminal structure contains a glycine fingerprint sequence Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
D13A lower turnover and lower Km than wild-type Staphylococcus aureus
D332A lower turnover and higher Km than wild-type Staphylococcus aureus
H125A higher turnover and lower Km than wild-type Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 UDPglucose H125A Staphylococcus aureus
1.1
-
 UDPglucose mutant D13A Staphylococcus aureus
1.3
-
 UDPglucose wild-type Staphylococcus aureus
3.3
-
 UDPglucose mutant D332A Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + (1,3-beta-D-glucosyl)n
-
 Staphylococcus aureus UDP + (1,3-beta-D-glucosyl)n+1
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00217
-
 UDPglucose D332A Staphylococcus aureus
0.0433
-
 UDPglucose D13A Staphylococcus aureus
0.55
-
 UDPglucose wild-type Staphylococcus aureus
0.717
-
 UDPglucose H125A Staphylococcus aureus