Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | competitive with respect to UDP-galactose | Homo sapiens | |
UDP-N-acetylgalactosamine | weak, competitive with respect to UDP-galactose | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
UDP-galactose | - |
Homo sapiens | |
0.5 | - |
2'-fucosyllactose | - |
Homo sapiens | |
2.2 | - |
O-alpha-L-fucosyl-1,2-galactose | - |
Homo sapiens | |
2.5 | - |
lacto-N-fucopentaose I | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight activation | Homo sapiens | |
Mn2+ | divalent cation required, Mn2+ most effective, optimum concentration 20 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
blood group B donors | - |
Purification (Comment) | Organism |
---|---|
one-step procedure involving absorption onto group 0 erythrocyte membranes followed by elution with the low molecular weight H-active trisaccharide 2'-fucosyllactose | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | - |
Homo sapiens | - |
stomach | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.92 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + 2'-fucosyllactose | - |
Homo sapiens | UDP + alpha-D-galactosyl-1,3-[2'-fucosyllactose] | - |
? | |
UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose | enzyme transfers D-galactose in alpha-linkage to oligosaccharides, glycolipids and glycoproteins with terminal non-reducing H-active structures and confers blood group B activity on group 0 erythrocytes | Homo sapiens | UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose | - |
? | |
UDP-galactose + H-active glycoprotein | - |
Homo sapiens | UDP + B-active substance | - |
? | |
UDP-galactose + lacto-N-fucopentaose I | - |
Homo sapiens | UDP + alpha-D-galactosyl-1,3-[lacto-N-fucopentaose I] | - |
? | |
UDP-galactose + O-alpha-L-fucosyl-1,2-galactose | - |
Homo sapiens | UDP + alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactopyranoside | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, 20% loss of activity, 20 min, 60% loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |