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Literature summary for 2.4.1.37 extracted from

  • Sindhuwinata, N.; Munoz, E.; Munoz, F.J.; Palcic, M.M.; Peters, H.; Peters, T.
    Binding of an acceptor substrate analog enhances the enzymatic activity of human blood group B galactosyltransferase (2010), Glycobiology, 20, 718-723.
    View publication on PubMed
Search for more literature for 2.4.1.37

Activating Compound

Activating Compound Comment Organism Structure
alpha-L-Fuc-(1,2)-beta-D-3-deoxy-Gal-O-octyl i.e. 3DD, an acceptor substrate analogue, accelerates enzymatic hydrolysis of UDP-Gal Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, GTB requires divalent metal ions for full activity Homo sapiens
Mn2+ activates 9fold at 24°C, GTB requires divalent metal ions for full activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose Homo sapiens i.e. H disaccharide UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose
-
 ?
UDP-galactose + blood group antigen Homo sapiens GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors UDP + alpha-D-galactosyl-blood group antigen
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by cation exchange chromatograophy, UDP-hexanolamine affinity chromatography, and dialysis Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose i.e. H disaccharide Homo sapiens UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose
-
 ?
UDP-galactose + blood group antigen GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors Homo sapiens UDP + alpha-D-galactosyl-blood group antigen
-
 ?
UDP-galactose + blood group antigen H GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors Homo sapiens UDP + alpha-D-galactosyl-blood group antigen H
-
 ?

Synonyms

Synonyms Comment Organism
blood group B galactosyltransferase
-
 Homo sapiens
GTB
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function human blood group B galactosyltransferase responsible for the biosynthesis of human blood group antigens Homo sapiens