Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant M340H enzymes in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified wild-type and M340H mutant enzymes in complex with pentasaccharide GlcNAcbeta1,2-Manalpha1,6 (GlcNAcbeta1,2-Manalpha1,3) and trisaccharide GlcNAcbeta1,4-GlcNAcbeta1,4-GlcNAc substrates, X-ray diffraction structure determination and analysis at 1.9-2.0 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M340H | site-directed mutagenesis, substrate binding structure in comparison to the wild-type enzyme, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, implications of the oligosaccharide structures on the kinetic parameters, overview | Homo sapiens | |
0.01 | - |
N-acetyl-beta-D-glucosamine | - |
Homo sapiens | |
0.06 | - |
GlcNAcbeta1,2-Manalpha1,6-Manbeta-OR | - |
Homo sapiens | |
0.6 | - |
GlcNAcbeta1,2-Manalpha1,3-Manbeta-OR | - |
Homo sapiens | |
0.71 | - |
GlcNAcbeta1,4-Manalpha1,3-Manbeta-OR | - |
Homo sapiens | |
0.96 | - |
chitotriose | - |
Homo sapiens | |
1.23 | - |
GlcNAcbeta1,2-Manalpha1,6 (GlcNAcbeta1,2-Manalpha1,3)Man | - |
Homo sapiens | |
1.59 | - |
N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide | Homo sapiens | the enzyme transfers galactose from UDP-galactose to N-acetylglucosamine residues of the branched N-linked oligosaccharide chains of glycoproteins | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15291 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | complex penta-antennary N-glycan structure, detailed substrate binding structure analysis of the wild-type and mutant M340H enzymes, overview | Homo sapiens | ? | - |
? | |
UDP-galactose + chitotriose | GlcNAcbeta1,4-GlcNAcbeta1,4-GlcNAc | Homo sapiens | ? | - |
? | |
UDP-galactose + GlcNAcbeta1,2-Manalpha1,3-Manbeta-OR | an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,2-1,3-arm) group of mannose, which is beta-1,4-linked to an N-linked chitobiose, attached to the aspargine residue of a protein | Homo sapiens | ? | - |
? | |
UDP-galactose + GlcNAcbeta1,2-Manalpha1,6 (GlcNAcbeta1,2-Manalpha1,3)Man | - |
Homo sapiens | ? | - |
? | |
UDP-galactose + GlcNAcbeta1,2-Manalpha1,6-Manbeta-OR | an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,2-1,6-arm) group of mannose, which is beta-1,4-linked to an N-linked chitobiose, attached to the aspargine residue of a protein | Homo sapiens | ? | - |
? | |
UDP-galactose + GlcNAcbeta1,4-Manalpha1,3-Manbeta-OR | an N-linked biantennary oligosaccharide chain, one antenna is attached to the 3-hydroxyl-(1,4-1,3-arm) group of mannose, which is beta-1,4-linked to an N-linked chitobiose, attached to the aspargine residue of a protein | Homo sapiens | ? | - |
? | |
UDP-galactose + N-acetyl-beta-D-glucosamine | - |
Homo sapiens | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
UDP-galactose + N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine | - |
Homo sapiens | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine | - |
? | |
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide | - |
Homo sapiens | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? | |
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide | the enzyme transfers galactose from UDP-galactose to N-acetylglucosamine residues of the branched N-linked oligosaccharide chains of glycoproteins | Homo sapiens | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase-I | - |
Homo sapiens |
beta4Gal-T1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.74 | - |
GlcNAcbeta1,2-Manalpha1,6 (GlcNAcbeta1,2-Manalpha1,3)Man | - |
Homo sapiens | |
1.9 | - |
GlcNAcbeta1,2-Manalpha1,6-Manbeta-OR | - |
Homo sapiens | |
2.47 | - |
GlcNAcbeta1,2-Manalpha1,3-Manbeta-OR | - |
Homo sapiens | |
3.3 | - |
chitotriose | - |
Homo sapiens | |
3.35 | - |
GlcNAcbeta1,4-Manalpha1,3-Manbeta-OR | - |
Homo sapiens | |
3.6 | - |
N-acetyl-beta-D-glucosamine | - |
Homo sapiens | |
4.81 | - |
chitotriose | - |
Homo sapiens | |
4.82 | - |
N-acetyl-beta-D-glucosaminyl-1,4-N-acetyl-beta-D-glucosamine | - |
Homo sapiens |