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Literature summary for 2.4.1.4 extracted from

  • Seo, D.H.; Jung, J.H.; Jung, D.H.; Park, S.; Yoo, S.H.; Kim, Y.R.; Park, C.S.
    An unusual chimeric amylosucrase generated by domain-swapping mutagenesis (2016), Enzyme Microb. Technol., 86, 7-16.
    View publication on PubMed
Search for more literature for 2.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene dgas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Deinococcus geothermalis
gene npas, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Neisseria polysaccharea

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric enzymes using gene dgas and gene npas from Deinococcus geothermalis by overlap extension polymerase chain reaction, the mutants show altered polymerization activity and thermostability Neisseria polysaccharea
additional information construction of chimeric enzymes using gene dgas and gene npas from Neisseria polysaccharea by overlap extension polymerase chain reaction, the mutants show altered polymerization activity and thermostability. Three-dimensional modeling structure and molecular dynamics of mutant DGAS-B, quaternary structure Deinococcus geothermalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Neisseria polysaccharea amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate ?
-
 ?
additional information Deinococcus geothermalis amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Deinococcus geothermalis Q1J0W0 gene dgas
-
Neisseria polysaccharea Q9ZEU2 gene npas
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Neisseria polysaccharea
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Deinococcus geothermalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate Neisseria polysaccharea ?
-
 ?
additional information amylosucrase synthesizes alpha-1,4-glucans using sucrose as a sole substrate Deinococcus geothermalis ?
-
 ?

Subunits

Subunits Comment Organism
More the active pocket in the enzyme is surrounded by A, B, and B' domains. The A domain serves as a catalytic domain and has the typical (beta/alpha)8-barrel structure of the GH13 family. The B' domain includes oligosaccharide binding sites, and both the B and B' domains of the enzyme contain acceptor subsites (+2, +3, +4, +5, and +6) Deinococcus geothermalis
More the active pocket in the enzyme is surrounded by A, B, and B' domains. The A domain serves as a catalytic domain andhas the typical (beta/alpha)8-barrel structure of the GH13 family. The B' domain includes oligosaccharide binding sites, and both the B and B' domains of the enzyme contain acceptor subsites (+2, +3, +4, +5, and +6) Neisseria polysaccharea

Synonyms

Synonyms Comment Organism
ASASE
-
 Neisseria polysaccharea
ASASE
-
 Deinococcus geothermalis
DGAS
-
 Deinococcus geothermalis
NPAS
-
 Neisseria polysaccharea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 55 recombinant chimeric enzymes Neisseria polysaccharea
30 55 recombinant chimeric enzymes Deinococcus geothermalis
40
-
 assay at, wild-type enzyme Deinococcus geothermalis
45
-
 assay at, wild-type enzyme Neisseria polysaccharea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43.2 51.5 the half-lives of mutant NPAS-B' and wild-type NPAS are 4.28 and 9.99 min at 45°C and their melting temperatures are 43.25°C and 51.52°C, respectively Neisseria polysaccharea
50 62 half-lives of mutant DGAS-B are 30.70 and 3.39 min at 50°C and 55°C, respectively, whereas that of the wild-type DGAS is 65.74 min at 55°C. The melting temperatures of mutant DGAS-B and wild-type DGAS are 54.23°C and 61.42°C, respectively Deinococcus geothermalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 wild-type enzyme Deinococcus geothermalis
7
-
 recombinant chimeric enzyme DGAS-B Deinococcus geothermalis
8
-
 recombinant chimeric enzyme NPAS-B' Neisseria polysaccharea
8 9 wild-type enzyme Neisseria polysaccharea

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 activity range, wild-type and mutant enzymes, profile overview Neisseria polysaccharea
5.5 9.5 activity range, wild-type and mutant enzymes, profile overview Deinococcus geothermalis