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Literature summary for 2.4.1.41 extracted from

  • Sugiura, M.; Kawasaki, T.; Yamashina, I.
    Purification and characterization of UDP-GalNAc:polypeptide N-acetylgalactosamine transferase from an ascites hepatoma, AH 66 (1982), J. Biol. Chem., 257, 9501-9507.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete inhibition Rattus norvegicus
additional information not inhibited by ATP, CDP, GDP Rattus norvegicus
UDP competitive Rattus norvegicus
UMP uncompetitive Rattus norvegicus
UTP competitive Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0417
-
UDP-GalNAc
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ less stimulation than by Mn2+ Rattus norvegicus
Cu2+ less stimulation than by Mn2+ Rattus norvegicus
Mn2+ requirement Rattus norvegicus
Mn2+ most effective cation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
gel filtration Rattus norvegicus
56000
-
1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
female albino rats
-

Purification (Commentary)

Purification (Comment) Organism
48100fold Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma ascites cell AH-66 ascites hepatoma cells, harvested 6-7 days after the intraperitoneal transfer of ascites fluid from donor rats, activity much higher than in rat liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.39
-
-
Rattus norvegicus
2.12
-
purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 3 months, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: lacto-N-fucopentaose I, ceramide dihexosides, ceramide trihexosides, globoside Rattus norvegicus ?
-
?
UDP-N-acetyl-D-galactosamine + A1 protein
-
Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-A1 protein
-
?
UDP-N-acetyl-D-galactosamine + apoantifreeze glycoproteins apoantifreeze glycoprotein of high molecular weight accepts GalNAc much faster than apoantifreeze glycoprotein 8 Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-apoantifreeze glycoproteins
-
?
UDP-N-acetyl-D-galactosamine + apofetuin lowest rate of transfer Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-apofetuin
-
?
UDP-N-acetyl-D-galactosamine + apomucin enzymatically prepared apomucin accepts GalNAc much faster than chemically prepared apomucin Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-apomucin
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
-
Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
?
UDP-N-acetyl-D-galactosamine + polypeptide only UDP-GalNAc serves as sugar donor Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?
UDP-N-acetyl-D-galactosamine + polypeptide acceptors: apomucin, kappa-casein Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?
UDP-N-acetyl-D-galactosamine + polypeptide acceptors: A1 protein, apofetuin, apoantifreeze glycoproteins, frequent glycosylation of the abundant serine plus threonine residues of mucins Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
UDP
-
Rattus norvegicus
0.0835
-
UTP
-
Rattus norvegicus
0.347
-
UMP
-
Rattus norvegicus