Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inhibition | Rattus norvegicus | |
additional information | not inhibited by ATP, CDP, GDP | Rattus norvegicus | |
UDP | competitive | Rattus norvegicus | |
UMP | uncompetitive | Rattus norvegicus | |
UTP | competitive | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0417 | - |
UDP-GalNAc | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | less stimulation than by Mn2+ | Rattus norvegicus | |
Cu2+ | less stimulation than by Mn2+ | Rattus norvegicus | |
Mn2+ | requirement | Rattus norvegicus | |
Mn2+ | most effective cation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
gel filtration | Rattus norvegicus |
56000 | - |
1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female albino rats | - |
Purification (Comment) | Organism |
---|---|
48100fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma ascites cell | AH-66 ascites hepatoma cells, harvested 6-7 days after the intraperitoneal transfer of ascites fluid from donor rats, activity much higher than in rat liver | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.39 | - |
- |
Rattus norvegicus |
2.12 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 3 months, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: lacto-N-fucopentaose I, ceramide dihexosides, ceramide trihexosides, globoside | Rattus norvegicus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + A1 protein | - |
Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-A1 protein | - |
? | |
UDP-N-acetyl-D-galactosamine + apoantifreeze glycoproteins | apoantifreeze glycoprotein of high molecular weight accepts GalNAc much faster than apoantifreeze glycoprotein 8 | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-apoantifreeze glycoproteins | - |
? | |
UDP-N-acetyl-D-galactosamine + apofetuin | lowest rate of transfer | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-apofetuin | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | enzymatically prepared apomucin accepts GalNAc much faster than chemically prepared apomucin | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + kappa-casein | - |
Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-kappa-casein | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | only UDP-GalNAc serves as sugar donor | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: apomucin, kappa-casein | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: A1 protein, apofetuin, apoantifreeze glycoproteins, frequent glycosylation of the abundant serine plus threonine residues of mucins | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
UDP | - |
Rattus norvegicus | |
0.0835 | - |
UTP | - |
Rattus norvegicus | |
0.347 | - |
UMP | - |
Rattus norvegicus |