KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
Arg-Thr-Pro-Pro-Pro | derived from the major acceptor sequence in myelin basic protein | Bos taurus | |
6 | - |
Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Bos taurus | catalyzes in vivo glycosylation of both threonine and serine | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
colostrum | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: bovine cytochrome c | Bos taurus | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + Arg-Thr-Pro-Pro-Pro | - |
Bos taurus | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly | - |
Bos taurus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | bovine apomucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + myelin basic protein | - |
Bos taurus | UDP + N-acetyl-D-galactosaminyl-myelin basic protein | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | broad specificity, acceptors: unglycosylated protein acceptors, synthetic peptides, O-glycosylates threonine about 35times faster than serine, specificity is determined by the amino acids in the acceptor peptide segment and its accessibility, enzymatic active site interacts with an 8-amino acid long segment of the substrate, spanning 3 amino acids preceding and 4 amino acids following the reactive Thr or Ser | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | catalyzes in vivo glycosylation of both threonine and serine | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + rhodanese | reduced and carboxylmethylated bovine rhodanese, at a lower rate than with myelin basic protein | Bos taurus | UDP + N-acetyl-D-galactosaminyl-rhodanese | - |
? | |
UDP-N-acetyl-D-galactosamine + subtilisin | reduced and carboxylmethylated bacterial subtilisin, at a lower rate than with myelin basic protein or bovine rhodanese | Bos taurus | UDP + N-acetyl-D-galactosaminyl-subtilisin | - |
? |