Cloned (Comment) | Organism |
---|---|
deletion mutant GalNAc-T1 cDNA is cloned and expressed in COS7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D444A | GalNAc-T1 mutant with severely impaired apomucin glycosylation, D444A/D484A/D525A triple mutant has significantly lower activity than D444A single mutant | Rattus norvegicus |
F468A | GalNAc-T1 mutant with strongly reduced activity and decreased expression | Rattus norvegicus |
G455Q | GalNAc-T1 mutant with reduced reactivity towards apomucin | Rattus norvegicus |
additional information | site-directed mutagenesis at the C-terminus and its deletion inactivates GalNAc-T1, probably caused by conformational changes in the (QXW)3 repeats, deletion of the lectin domain results in complete loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GalNAc | inhibition of GalNAc-T1 by free GalNAc: follow-up O-glycosylation of apomucin is sensitive, O-glycosylation of synthetic peptides containing a single glycosylation site is resistant | Rattus norvegicus | |
additional information | GalNAc-T1: follow-up glycosylation is inhibited by addition of beta1,3Gal to GalNAc, apomucin glycosylation is not inhibited by galactose, glucose, N-acetylglucosamine, mannose and fucose | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | increased values, mg/ml, for apomucin of several GalNAc-T1 mutants | Rattus norvegicus | |
0.2 | - |
Pro-Pro-Asp-Ala-Ala-Thr-Ala-Ala-Pro-Leu | GalNAc-T1 | Rattus norvegicus | |
0.8 | - |
Gly-Val-Val-Pro-Thr-Val-Val-Pro-Gly | GalNAc-T1 | Rattus norvegicus | |
3.6 | - |
UDP-GalNAc | GalNac-T1 mutant N465A | Rattus norvegicus | |
4 | - |
UDP-GalNAc | GalNac-T1 mutant G455Q | Rattus norvegicus | |
4.4 | - |
UDP-GalNAc | GalNac-T1 triple mutant D444A/D484A/D525A | Rattus norvegicus | |
5.1 | - |
UDP-GalNAc | GalNac-T1 mutants D444A and P-deltaN42 | Rattus norvegicus | |
5.4 | - |
UDP-GalNAc | GalNac-T1 mutant F457A | Rattus norvegicus | |
6.1 | - |
UDP-GalNAc | GalNac-T1 mutant Q466A | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | type II membrane protein | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | essential for donor substrate binding | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Rattus norvegicus | GalNAc-T1 glycosylates unmodified polypeptides in vivo | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Rattus norvegicus | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
GalNAc-T1 | - |
Purification (Comment) | Organism |
---|---|
recombinant mutant GalNAc-T1, expressed in COS7 cells | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | contains a lectin-like repeat sequence at the C-terminus, role of GalNAc-T1 lectin domain, which is involved in glycosylation of substrates with multiple acceptor sites | Rattus norvegicus | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + Gly-Val-Val-Pro-Thr-Val-Val-Pro-Gly | - |
Rattus norvegicus | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Thr-Ala-Ala-Pro-Leu | - |
Rattus norvegicus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | deglycosylated bovine submaxillary mucin | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | GalNAc-T1 | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide | GalNAc-T1 may also specifically recognize and glycosylate partially glycosylated acceptors | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | GalNAc-T1: Asp-144 is the most important site for GalNAc recognition, primarily recognizes the UDP portion of UDP-GalNAc | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | GalNAc-T1 glycosylates unmodified polypeptides in vivo | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
68 | - |
GalNAc | inhibition of GalNAc-T1 mutant P-deltaN42 | Rattus norvegicus | |
175 | - |
GalNAc | inhibition of GalNAc-T1 mutant D444A | Rattus norvegicus |