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Literature summary for 2.4.1.43 extracted from
- Successive glycosyltransfer activity and enzymatic characterization of pectic polygalacturonate 4-alpha-galacturonosyltransferase solubilized from pollen tubes of Petunia axillaris using pyridylaminated oligogalacturonates as substrates (2002), Plant Physiol., 130, 374-379.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
fluorogenic pyridylaminated-oligogalacturonic acid | degree of polymerization: 14 | Petunia axillaris | |
| 0.013 | - |
fluorogenic pyridylaminated-oligogalacturonic acid | degree of polymerization: 11 | Petunia axillaris | |
| 0.044 | - |
fluorogenic pyridylaminated-oligogalacturonic acid | degree of polymerization: 7 | Petunia axillaris | |
| 0.17 | - |
UDP-D-galacturonic acid | - |
Petunia axillaris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 20% of activation with Mn2+ | Petunia axillaris | |
| CoCl2 | - |
Petunia axillaris | |
| MgCl2 | 5 mM, 20% of activation with Mn2+ | Petunia axillaris | |
| MnCl2 | 5-10 mM, maximal activation, no activity without divalent cation | Petunia axillaris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petunia axillaris | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen tube | - |
Petunia axillaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-galacturonic acid + fluorogenic pyridylaminated-oligogalacturonic acid | degree of polymerization 7-14, galacturonic acid is transferred onto the nonreducing end of fluorogenic pyridylaminated-oligogalacturonic acid through alpha-1,4-linkages | Petunia axillaris | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Petunia axillaris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | approx. 40% of maximal activity at 10 and 40°C | Petunia axillaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
substrate fluorogenic pyridylaminated-oligogalacturonic acid | Petunia axillaris |
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Release 2023.1 (January 2023)
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