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Literature summary for 2.4.1.46 extracted from

  • Marechal, E.; Miege, C.; Block, M.A.; Douce, R.; Joyard, J.
    The catalytic site of monogalactosyldiacylglycerol synthase from spinach chloroplast envelope membranes. Biochemical analysis of the structure and of the metal content (1995), J. Biol. Chem., 270, 5714-5722.
    View publication on PubMed
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General Stability

General Stability Organism
dithiothreitol is essential to prevent loss of activity during purification Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
citraconic anhydride 0.01 and 0.05 mM, 47% and 97% inactivation after 30 min, respectively Spinacia oleracea
N-ethylmaleimide 5 mM, complete inactivation of enzyme in envelope membranes, 0.008 mM, complete inactivation of solubilized enzyme, preincubation with 1,2-dioleoylglycerol protects Spinacia oleracea
o-phenanthroline 0.1 mM, 55% inhibition, 1,2-dioleoylglycerol protects and activates enzyme Spinacia oleracea
tert-butyoxycarbonyl-L-methionine hydrosuccinimidyl ester 0.2 mM, almost complete inactivation after 30 min Spinacia oleracea
UDP
-
 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast envelope
-
 Spinacia oleracea 9941
-

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
 UDP UDPgalactose as the varied substrate Spinacia oleracea
0.052
-
 UDP dioleoylglycerol as the varied substrate Spinacia oleracea