General Stability | Organism |
---|---|
freezing causes 80-85% loss of activity, 2-mercaptoethanol protects against inactivation by freezing | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-sphingosine | at higher concentrations | Gallus gallus | |
DL-sphingosine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
UDPgalactose | - |
Gallus gallus | |
0.11 | - |
2-(2-hydroxyacyl)sphingosine | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates at 10 mM, dialyzed enzyme has requirement for divalent cations | Gallus gallus | |
Mg2+ | stimulates | Gallus gallus | |
Mg2+ | stimulates | Rattus norvegicus | |
Mg2+ | dialyzed enzyme has requirement for divalent cations, optimal stimulation at 5 mM | Gallus gallus | |
Mn2+ | stimulates | Gallus gallus | |
Mn2+ | stimulates | Rattus norvegicus | |
Mn2+ | at 10 mM, dialyzed enzyme has requirement for divalent cations | Gallus gallus | |
additional information | not activated by Cu2+, Fe2+, Co2+, Ni2+, Zn2+, Cd2+ | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | Gallus gallus | i.e. 2-hydroxy fatty acid ceramide, synthesis of cerebroside | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | - |
? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | Gallus gallus | enzyme may play an important role in the synthesis of at least one type of myelin | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
3 days old | - |
Gallus gallus | - |
19-20 days old embryos | - |
Rattus norvegicus | - |
1 month old | - |
Sus scrofa | - |
fetus | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Gallus gallus | - |
brain | - |
Cavia porcellus | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Sus scrofa | - |
embryo | maximal activity in 19-20 day-old embryos | Gallus gallus | - |
Storage Stability | Organism |
---|---|
4°C, dialyzed enzyme, 0.1% 2-mercaptoethanol, 0.32 M sucrose, 7 days, stable | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Rattus norvegicus | ? | - |
? | |
additional information | not: galactose, galactose-1-phosphat, ADP-galactose | Gallus gallus | ? | - |
? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | - |
Rattus norvegicus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | i.e. 2-hydroxy fatty acid galactosylceramide | ? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | highly specific | Gallus gallus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | product: galactocerebroside containing 2-hydroxy fatty acids linked to the amino group of the sphingosine moiety | ? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | ceramide containing 2-hydroxystearic acid as the predominant fatty acid | Gallus gallus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | product: galactocerebroside containing 2-hydroxy fatty acids linked to the amino group of the sphingosine moiety | ? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | i.e. 2-hydroxy fatty acid ceramide, synthesis of cerebroside | Gallus gallus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | - |
? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | enzyme may play an important role in the synthesis of at least one type of myelin | Gallus gallus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | - |
? | |
UDPglucose + 2-(2-hydroxyacyl)sphingosine | at 7-10% of the activity with UDP-galactose | Gallus gallus | glucocerebroside + UDP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
in bicine buffer | Gallus gallus |