Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.48 extracted from

  • Ankel, H.; Ankel, E.; Schutzbach, J.S.
    Mannosyl transfer in Cryptococcus laurentii (1970), J. Biol. Chem., 245, 3945-3955.
    View publication on PubMed
Search for more literature for 2.4.1.48

General Stability

General Stability Organism
repeated freezing and thawing reduces activity Cryptococcus laurentii var. flavescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 GDP-mannose
-
 Cryptococcus laurentii var. flavescens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cell wall associated Cryptococcus laurentii var. flavescens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20 mM, required, 15% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
Co2+ 20 mM, required, 16% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
Cu2+ 20 mM, required, about 1% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
Mg2+ 20 mM, required, 35% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
Mn2+ 20 mM, required, 100% activity Cryptococcus laurentii var. flavescens
additional information absolute requirement for a divalent cation Cryptococcus laurentii var. flavescens
Ni2+ 20 mM, required, 4% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
Zn2+ 20 mM, required, 16% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + heteroglycan Cryptococcus laurentii var. flavescens
-
 GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?
GDP-mannose + heteroglycan Cryptococcus laurentii var. flavescens NRRL Y-1401
-
 GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?

Organism

Organism UniProt Comment Textmining
Cryptococcus laurentii var. flavescens
-
 NRRL Y-1401
-
Cryptococcus laurentii var. flavescens NRRL Y-1401
-
 NRRL Y-1401
-

Purification (Commentary)

Purification (Comment) Organism
partial Cryptococcus laurentii var. flavescens

Storage Stability

Storage Stability Organism
-20°C, stable for at least 4 weeks Cryptococcus laurentii var. flavescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + heteroglycan
-
 Cryptococcus laurentii var. flavescens GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?
GDP-mannose + heteroglycan
-
 Cryptococcus laurentii var. flavescens NRRL Y-1401 GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?
GDP-mannose + heteropolysaccharide heteropolysaccharide containing mannosyl, galactosyl, xylosyl units Cryptococcus laurentii var. flavescens GDP + 1,2(or 1,3)-alpha-D-mannosylheteropolysaccharide
-
 ?
GDP-mannose + heteropolysaccharide heteropolysaccharide containing mannosyl, galactosyl, xylosyl units Cryptococcus laurentii var. flavescens NRRL Y-1401 GDP + 1,2(or 1,3)-alpha-D-mannosylheteropolysaccharide
-
 ?
additional information mannose-1-phosphate and mannose are no substrates Cryptococcus laurentii var. flavescens ?
-
 ?
additional information mannose-1-phosphate and mannose are no substrates Cryptococcus laurentii var. flavescens NRRL Y-1401 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Cryptococcus laurentii var. flavescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Cryptococcus laurentii var. flavescens

pH Range

pH Minimum pH Maximum Comment Organism
6 10 20% of maximal activity at pH 6 and pH 10 Cryptococcus laurentii var. flavescens