Application | Comment | Organism |
---|---|---|
biotechnology | potential application of the C-terminal enzyme domain GBD-7 as an affinity tag onto cheap resins like for rapid purification of dextrans | Leuconostoc mesenteroides |
Cloned (Comment) | Organism |
---|---|
expression of thioredoxin-His-tagged wild-type and trunacted mutant C-terminal domain GBD-7 | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of fourteen truncated forms of strain NRRL B512-F dextransucrase by N-, C- or N- plus C-terminal domain truncations, dextran binding properties of mutant enzymes, overview | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides NRRL B512-F | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant thioredoxin-His-tagged wild-type and truncated mutant C-terminal domain GBD-7 about 6fold by nickel affinity chromatography | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
dextran binding capacity of recombinant wild-type and truncated mutant C-terminal domain GBD-7, overview | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + dextran | glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + dextran | glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview | Leuconostoc mesenteroides NRRL B512-F | ? | - |
? | |
sucrose + isomaltohexaose | glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + isomaltohexaose | glucose transfer reaction, determination of the dextransucrase minimal motif involved in dextran binding, strong interaction with dextran is localized between amino acids N1397 and A1527 of the C-terminal domain, GBD-7, and consists of six YG repeats, overview. The motif containing enzyme shows very high affinity for isomaltohexaose and longer dextrans and is involved in polymer formation, overview | Leuconostoc mesenteroides NRRL B512-F | ? | - |
? |