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Literature summary for 2.4.1.5 extracted from

  • Kang, H.K.; Kim, Y.M.; Kim, D.M.
    Functional, genetic, and bioinformatic characterization of dextransucrase (DSRBCB4) gene in Leuconostoc mesenteroides B-1299CB4 (2008), J. Microbiol. Biotechnol., 18, 1050-1058.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene dsrBCB4, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
D530N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Leuconostoc mesenteroides
D641N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Leuconostoc mesenteroides
E568Q site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Leuconostoc mesenteroides
EDTA
-
Leuconostoc mesenteroides
Fe2+
-
Leuconostoc mesenteroides
Hg2+
-
Leuconostoc mesenteroides
Zn2+
-
Leuconostoc mesenteroides

Metals/Ions

Metals/Ions Comment Organism Structure
additional information DSRBCB4 activity is not affected by Mg2+, K+, or Na+ Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
gene dsrBCB4
-
Leuconostoc mesenteroides B-1299CB4
-
gene dsrBCB4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes about 6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
mutant activities Leuconostoc mesenteroides
13.9
-
purified recombinant wild-type DSRBCB4 Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the dextransucrase DSRBCB4 synthesizes only alpha-1,6-linked dextran, the recombinantly expressed DSRBCB4 synthesizes oligosaccharides in the presence of maltose or isomaltose as an acceptor, the products including alpha-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue, conserved amino acid residues in the catalytic core, D530, E568, and D641, that are critical for enzyme activity, NMR product analysis, overview Leuconostoc mesenteroides ?
-
?
additional information the dextransucrase DSRBCB4 synthesizes only alpha-1,6-linked dextran, the recombinantly expressed DSRBCB4 synthesizes oligosaccharides in the presence of maltose or isomaltose as an acceptor, the products including alpha-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue, conserved amino acid residues in the catalytic core, D530, E568, and D641, that are critical for enzyme activity, NMR product analysis, overview Leuconostoc mesenteroides B-1299CB4 ?
-
?

Synonyms

Synonyms Comment Organism
DSRBCB4
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Leuconostoc mesenteroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 30 62% of maximal activity at 15°C Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
Leuconostoc mesenteroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 80% remaining activity, rapid decrease outside this range Leuconostoc mesenteroides