Cloned (Comment) | Organism |
---|---|
gene dsrBCB4, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
D530N | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
D641N | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
E568Q | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Leuconostoc mesenteroides | |
EDTA | - |
Leuconostoc mesenteroides | |
Fe2+ | - |
Leuconostoc mesenteroides | |
Hg2+ | - |
Leuconostoc mesenteroides | |
Zn2+ | - |
Leuconostoc mesenteroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | DSRBCB4 activity is not affected by Mg2+, K+, or Na+ | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
gene dsrBCB4 | - |
Leuconostoc mesenteroides B-1299CB4 | - |
gene dsrBCB4 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes about 6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant activities | Leuconostoc mesenteroides |
13.9 | - |
purified recombinant wild-type DSRBCB4 | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the dextransucrase DSRBCB4 synthesizes only alpha-1,6-linked dextran, the recombinantly expressed DSRBCB4 synthesizes oligosaccharides in the presence of maltose or isomaltose as an acceptor, the products including alpha-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue, conserved amino acid residues in the catalytic core, D530, E568, and D641, that are critical for enzyme activity, NMR product analysis, overview | Leuconostoc mesenteroides | ? | - |
? | |
additional information | the dextransucrase DSRBCB4 synthesizes only alpha-1,6-linked dextran, the recombinantly expressed DSRBCB4 synthesizes oligosaccharides in the presence of maltose or isomaltose as an acceptor, the products including alpha-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue, conserved amino acid residues in the catalytic core, D530, E568, and D641, that are critical for enzyme activity, NMR product analysis, overview | Leuconostoc mesenteroides B-1299CB4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DSRBCB4 | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Leuconostoc mesenteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 30 | 62% of maximal activity at 15°C | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Leuconostoc mesenteroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | 80% remaining activity, rapid decrease outside this range | Leuconostoc mesenteroides |