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Literature summary for 2.4.1.5 extracted from

  • Yajie, W.; Hongbin, Z.; Xueqin, H.; Chunbao, Z.; Youjia, H.
    Purification and characterization of recombinant Escherichia coli dextransucrase (2008), Wei Sheng Wu Xue Bao, 48, 1266-1269.
    View publication on PubMed
Search for more literature for 2.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene dexYG, overexpression in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 1 mM Escherichia coli
SDS strong inhibition Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strong activation at 1 mM Escherichia coli
additional information little effect by 1 mM Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 x * 170000, recombinant enzyme, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene dexYG
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 11.4fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and nickel affinity chromatography Escherichia coli

Storage Stability

Storage Stability Organism
25°C, purified recombinant enzyme, 59% activity remaining after 4 days Escherichia coli
4°C, purified recombinant enzyme, 50% activity remaining after 7 weeks Escherichia coli

Subunits

Subunits Comment Organism
? x * 170000, recombinant enzyme, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 recombinant enzyme Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 instable above Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 recombinant enzyme Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 recombinant enzyme, quite stable Escherichia coli