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Literature summary for 2.4.1.5 extracted from

  • Amari, M.; Arango, L.F.; Gabriel, V.; Robert, H.; Morel, S.; Moulis, C.; Gabriel, B.; Remaud-Simeon, M.; Fontagne-Faucher, C.
    Characterization of a novel dextransucrase from Weissella confusa isolated from sourdough (2013), Appl. Microbiol. Biotechnol., 97, 5413-5422.
    View publication on PubMed
Search for more literature for 2.4.1.5

Application

Application Comment Organism
food industry the dextransucrase is responsible for production of dextran with predominant alpha-(1->6) linkages that might find applications as food hydrocolloids Weissella confusa

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Weissella cibaria
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged DSRC39-2 in Escherichia coli strain BL21(DE3) Weissella confusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information rDSRC39-2 shows Michaelis-Menten-type kinetics Weissella confusa
0.0086
-
 sucrose pH 5.4, 30°C, recombinant enzyme Weissella confusa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Weissella cibaria
-
-
soluble
-
 Weissella confusa
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Weissella cibaria
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa LBAE C39-2
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Weissella cibaria LBAE K39
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Weissella cibaria
-
 isolated from wheat sourdough, single copy gene
-
Weissella cibaria LBAE K39
-
 isolated from wheat sourdough, single copy gene
-
Weissella confusa A0A5E8F621 isolated from wheat sourdough, single copy gene
-
Weissella confusa LBAE C39-2 A0A5E8F621 isolated from wheat sourdough, single copy gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DSRC39-2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatogaphy Weissella confusa

Storage Stability

Storage Stability Organism
4°C, purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, about 10% activity loss after 6 days, and about 35% activity loss after 20 days Weissella confusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella cibaria D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella confusa LBAE C39-2 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Weissella cibaria LBAE K39 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

Synonyms Comment Organism
DSR
-
 Weissella cibaria
DSR
-
 Weissella confusa
DSRC39-2
-
 Weissella confusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Weissella confusa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, 30 min, stable, rapid loss of activity above 30°C Weissella confusa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
 sucrose pH 5.4, 30°C, recombinant enzyme Weissella confusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
 Weissella confusa

pI Value

Organism Comment pI Value Maximum pI Value
Weissella confusa sequence calculation
-
 4.6

General Information

General Information Comment Organism
evolution dextransucrases are glucansucrases belonging to the glycoside hydrolase family GH70 Weissella cibaria
evolution dextransucrases are glucansucrases belonging to the glycoside hydrolase family GH70 Weissella confusa
additional information the amino acids involved in catalysis, E592, D554, D665 are conserved in the catalytic core Weissella confusa
physiological function the dextransucrase is responsible for production of dextran with predominant alpha-(1->) linkages Weissella confusa