Application | Comment | Organism |
---|---|---|
synthesis | the strain Leuconostoc citreum strain B/110-1-2 is used for industrial production of dextran and dextran derivatives | Leuconostoc citreum |
Cloned (Comment) | Organism |
---|---|
two ORFs coding for strain B/110-1-2 dextransucrases, DNA and amino acid sequence determination and analysis, sequence comparisons | Leuconostoc citreum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leuconostoc citreum | - |
- |
intracellular | - |
Leuconostoc citreum | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | 180000 | intracellular dextransucrase 1 and extracellular dextransucrase | Leuconostoc citreum |
220000 | 240000 | intracellular dextransucrse 2 | Leuconostoc citreum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leuconostoc citreum | structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview | ? | - |
? | |
additional information | Leuconostoc citreum B/110-1-2 | structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview | ? | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Leuconostoc citreum | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Leuconostoc citreum B/110-1-2 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc citreum | D5FS20 | isolated from sugarcane juice | - |
Leuconostoc citreum B/110-1-2 | D5FS20 | isolated from sugarcane juice | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview | Leuconostoc citreum | ? | - |
? | |
additional information | structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview | Leuconostoc citreum B/110-1-2 | ? | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Leuconostoc citreum | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Leuconostoc citreum B/110-1-2 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leuconostoc citreum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
assay at | Leuconostoc citreum |
General Information | Comment | Organism |
---|---|---|
physiological function | in presence of sucrose, the strain B/110-1-2 produces two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46% of the total glucosyltransferase activity) at 160-180 kDa | Leuconostoc citreum |