Literature summary for 2.4.1.50 extracted from

Wang, C.; Kovanen, V.; Raudasoja, P.; Eskelinen, S.; Pospiech, H.; Myllylae, R.
The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability (2009), J. Cell. Mol. Med., 13, 508-521.

Search for more literature for 2.4.1.50

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in HT-1080 cells, expression of fragments in Escherichia coli	Homo sapiens
wild-type protein and His-tagged version expressed in fibrosarcoma cells HT-1080, 30 kDa His-tagged amino-terminal fragment (amino acid residue 25-290) with glycosyltransferase activity expressed in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A187D/A189D	30 kDa His-tagged amino-terminal fragment with mutations shows 2% glycosyltransferase activity of wild type fragment	Homo sapiens
additional information	LH-deficient mutant, deficiency of LH3 glycosyltransferase activity decreases cell growth and increases lethality	Homo sapiens
additional information	overexpression of LH3, increased hydroxylation of lysyl residues and increased galactosylation and glucosylation of hydroxylysyl residues	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens
Ni-NTA-agarose	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
CCD-19LU cell	-	Homo sapiens	-
embryonic kidney cell line	-	Homo sapiens	-
fibrosarcoma cell line	-	Homo sapiens	-
HEK-293 cell	-	Homo sapiens	-
HT-1080 cell	-	Homo sapiens	-
lung fibroblast cell line	-	Homo sapiens	-
MG-63 cell	-	Homo sapiens	-
osteosarcoma cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
hydroxylysyl galactosyltransferase	-	Homo sapiens
lysyl hydroxylase 3	-	Homo sapiens

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Release 2023.1 (January 2023)