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Literature summary for 2.4.1.65 extracted from

  • Palma, A.S.; Vila-Verde, C.; Pires, A.S.; Fevereiro, P.S.; Costa, J.
    A novel plant alpha4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewisa adhesion determinant (2001), FEBS Lett., 499, 235-238.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 20 mM, 98% inhibition Vaccinium myrtillus
Cu2+ 20 mM, complete inhibition Vaccinium myrtillus
Mn2+ 20 mM, 40% inhibition below pH 8.0 Vaccinium myrtillus
Zn2+ 20 mM, complete inhibition Vaccinium myrtillus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Vaccinium myrtillus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Homo sapiens
Mn2+ activation above pH 8.0 Vaccinium myrtillus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Vaccinium myrtillus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Vaccinium myrtillus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
-
Homo sapiens GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
?
GDP-L-fucose + 1,3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R transfer of fucose to N-acetylglucosamine in the type I Galbeta3GlcNAc motif from oligosaccharides linked to a hydrophobic tail and glycoproteins containing the type I motif. Sialylated oligosaccharides containing the type II Galbeta4GlcNAc motif are not acceptors Vaccinium myrtillus GDP + 1,3-beta-D-galactosyl-(alpha-1,4-L-fucosyl)-N-acetyl-D-glucosaminyl-R
-
?
GDP-L-fucose + asialofetuin
-
Homo sapiens GDP + ?
-
?
GDP-L-fucose + asialofetuin 7.3% of the activity with Galbeta3GlcNAc-O-sp-biotin Vaccinium myrtillus GDP + ?
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc-O-sp-biotin 118% of the activity with Galbeta3GlcNAc-O-sp-biotin Vaccinium myrtillus GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc-O-sp-biotin
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc-O-sp-biotin 10.5% of the activity with Galbeta3GlcNAc-O-sp-biotin Vaccinium myrtillus GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc-O-sp-biotin
-
?
GDP-L-fucose + Galbeta(1,3)GlcNAc-O-sp-biotin
-
Vaccinium myrtillus GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc-O-sp-biotin
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Vaccinium myrtillus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 50% of maximal activity, 50°C: about 40% of maximal activity Vaccinium myrtillus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Vaccinium myrtillus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
both in the presence and in absence of Mn2+ Vaccinium myrtillus