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Literature summary for 2.4.1.65 extracted from

  • Costa, J.; Grabenhorst, E.; Nimtz, M.; Conradt, H.S.
    Stable expression of the Golgi form and secretory variants of human fucosyltransferase III from BHK-21 cells. Purification and characterization of an engineered truncated form from the culture medium (1997), J. Biol. Chem., 272, 11613-11621.
    View publication on PubMed
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Application

Application Comment Organism
synthesis the soluble form of fucosyltransferase III secreted by stably transfected cells may be used for in vitro synthesis of the Lewis 1 determinant on carbohydrates and glycoproteins Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
construction of plasmids encoding soluble forms of the recombinant human FucT-III where the human IL-2 sequence is linked to Ala47 or Val-36 of the FucT-III and expression in stable transfected BHK-21 cell lines Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane stable BHK-21 cell lines express the Golgi bound form and two secretory forms of the enzyme Homo sapiens 16020
-
soluble stable BHK-21 cell lines express the Golgi bound form and two secretory forms of the enzyme. 40% of the enzyme activity synthesized by cells transfected with the Golgi form of the enzyme are constitutively secreted into the medium Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the secretory variant of enzyme contains N-linked endo H sensitive carbohydrate chains at its two glycosylation sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
secretory variant of enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + asialofetuin the acceptor oligosaccharide in bovine asialofetuin is the man3 branched triantennary isomer with one Galbeta(1,3)GlcNAc Homo sapiens GDP + ?
-
 ?
GDP-L-fucose + IL-4 receptor
-
 Homo sapiens GDP + ?
-
 ?
additional information the enzyme transferrs fucose to the O-4-position of GlcNAc in small oligosaccharides, glycolipids, glycopeptides and glycoproteins containing the type I Galbeta(1,3)GlcNAc motif Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000-42000, SDS-PAGE Homo sapiens