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Literature summary for 2.4.1.65 extracted from

  • Niu, X.; Fan, X.; Sun, J.; Ting, P.; Narula, S.; Lundell, D.
    Inhibition of fucosyltransferase VII by gallic acid and its derivatives (2004), Arch. Biochem. Biophys., 425, 51-57.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK 293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(-)-epigallocatechin-3-gallate time-dependent, irreversible Homo sapiens
2,3-Dihydroxybenzoic acid
-
Homo sapiens
2,4-dihydroxybenzoic acid
-
Homo sapiens
2,5-dihydroxybenzoic acid
-
Homo sapiens
3,4,5-Trihydroxybenzoic acid gallic acid, time-dependent, irreversible Homo sapiens
3,4,5-trimethoxybenzoic acid
-
Homo sapiens
3,4-dihydroxybenzoic acid
-
Homo sapiens
3,4-dimethoxybenzoic acid
-
Homo sapiens
3,5-dimethoxy 4-hydroxybenzoic acid syringic acid Homo sapiens
ellagic acid
-
Homo sapiens
methyl gallate
-
Homo sapiens
propyl gallate
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can replace Mn2+ for activation, optimum concentration is 10-15 mM Homo sapiens
Mg2+ can replace Mn2+ for activation, optimum concentration is 10-15 mM Homo sapiens
Mn2+ required for activation in vitro, optimum concentration is 10-15 mM Homo sapiens
additional information Cu2+ inactivates the enzyme Homo sapiens
Zn2+ can replace Mn2+ for activation, but shows only half the maximal activity, optimum concentration is 10-15 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q11130
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
fucosyltransferase 7
-
Homo sapiens
fucosyltransferase VII
-
Homo sapiens
FucT VII
-
Homo sapiens
FucT-VII
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information 2,3-dihydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 2,4-dihydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,5-dimethoxy 4-hydroxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,4-dimethoxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, 3,4,5-trimethoxybenzoic acid: above 0.200, presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.00006
-
3,4,5-Trihydroxybenzoic acid presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.0007
-
(-)-epigallocatechin-3-gallate presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.0012
-
ellagic acid presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.0022
-
(-)-epigallocatechin-3-gallate 60 min assay, no pre-incubation Homo sapiens
0.0022
-
(-)-epigallocatechin-3-gallate overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, MnCl2 omitted in the overnight pre-incubation mixture Homo sapiens
0.0054
-
3,4,5-Trihydroxybenzoic acid overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose, MnCl2 omitted in the overnight pre-incubation mixture Homo sapiens
0.008
-
3,4,5-Trihydroxybenzoic acid 60 min assay, no pre-incubation Homo sapiens
0.008
-
methyl gallate presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.045
-
propyl gallate presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.115
-
2,5-dihydroxybenzoic acid presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens
0.137
-
3,4-dihydroxybenzoic acid presence of MnCl2, overnight (15 h) pre-incubation of compound and enzyme before initiation of the reaction with GDP-fucose Homo sapiens