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Literature summary for 2.4.1.65 extracted from

  • Ma, B.; Audette, G.F.; Lin, S.; Palcic, M.M.; Hazes, B.; Taylor, D.E.
    Purification, kinetic characterization, and mapping of the minimal catalytic domain and the key polar goups of Helicobacter pylori alpha-(1,3/1,4)-fucosyltransferases (2006), J. Biol. Chem., 281, 6385-6394.
    View publication on PubMed
Search for more literature for 2.4.1.65

Cloned(Commentary)

Cloned (Comment) Organism
full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3 Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
additional information with full length UA948FucT, only 20% of total enzyme activities are localized in the soluble fraction. For UA948(1-428), this increases to 47%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. UA948(1-364), a mutant with the entire heptad repeat region removed, exhibits extremely low levels of enzyme activity. This indicates that the heptad repeat region is essential for enzyme activity. Construct UA948(1-371) has little enzyme activity. UA948(10-434) and UA948(1-434) completely lose activity Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0119
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, truncated enzyme UA948(1-428) Helicobacter pylori
0.0337
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, full-length enzyme Helicobacter pylori
5.7
-
 Galbeta1,3GlcNAc-O(CH2)8CO2CH3 truncated enzyme UA948(1-428) Helicobacter pylori
13.3
-
 Galbeta1,3GlcNAc-O(CH2)8CO2CH3 full-length enzyme Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51200
-
 51200, UA948FucT(1-428), calculated from sequence Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
 UA948
-
Helicobacter pylori UA948
-
 UA948
-

Purification (Commentary)

Purification (Comment) Organism
UA948FucT(1-428) with a His6 tag at the C terminus is purified Helicobacter pylori

Storage Stability

Storage Stability Organism
-80°C, 3 months, lyophilized UA948FucT(1-428) loses 23% of its activity Helicobacter pylori
most stable at -80°C with or without glycerol supplementation Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc 40% of the activity with Galbeta1,3GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc 40% of the activity with Galbeta1,3GlcNAc Helicobacter pylori NCTC11639 ?
-
 ?
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc 40% of the activity with Galbeta1,3GlcNAc Helicobacter pylori UA948 ?
-
 ?
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc 34% of the activity with Galbeta1,3GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc 34% of the activity with Galbeta1,3GlcNAc Helicobacter pylori NCTC11639 ?
-
 ?
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc 34% of the activity with Galbeta1,3GlcNAc Helicobacter pylori UA948 ?
-
 ?
GDP-beta-L-fucose + Galbeta1,3GlcNAc
-
 Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + Galbeta1,3GlcNAc
-
 Helicobacter pylori NCTC11639 ?
-
 ?
GDP-beta-L-fucose + Galbeta1,3GlcNAc
-
 Helicobacter pylori UA948 ?
-
 ?
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3
-
 Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3
-
 Helicobacter pylori NCTC11639 ?
-
 ?
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3
-
 Helicobacter pylori UA948 ?
-
 ?
additional information the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity Helicobacter pylori ?
-
 ?
additional information the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity Helicobacter pylori NCTC11639 ?
-
 ?
additional information the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity Helicobacter pylori UA948 ?
-
 ?

Subunits

Subunits Comment Organism
? 51200, UA948FucT(1-428), calculated from sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
alpha-(1,3/1,4)-fucosyltransferase
-
 Helicobacter pylori
alpha-(1,3/1,4)-FucT
-
 Helicobacter pylori
UA948FucT
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, truncated enzyme UA948(1-428) Helicobacter pylori
1.1
-
 Galbeta1,3GlcNAc-O(CH2)8CO2CH3 truncated enzyme UA948(1-428) Helicobacter pylori