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Literature summary for 2.4.1.66 extracted from

  • Wang, C.; Risteli, M.; Heikkinen, J.; Hussa, A.K.; Uitto, L.; Myllyla, R.
    Identification of amino acids important for the catalytic activity of the collagen glucosyltransferase associated with the multifunctional lysyl hydroxylase 3 (LH3) (2002), J. Biol. Chem., 277, 18568-18573.
    View publication on PubMed
Search for more literature for 2.4.1.66

Cloned(Commentary)

Cloned (Comment) Organism
cDNA sequence of GGT/LH3 Homo sapiens
LH cDNA with GGT activity is expressed in Escherichia coli XL1-Blue, cDNA sequence of GGT/LH Caenorhabditis elegans
LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue Homo sapiens

Protein Variants

Protein Variants Comment Organism
A361G mutant with reduced GGT activity Caenorhabditis elegans
A380V mutant with reduced GGT activity Caenorhabditis elegans
A453I mutant with a remarkably reduced GGT activity Caenorhabditis elegans
A464I no effect on GGT activity Homo sapiens
C132I mutant with dramatically reduced GGT activity Caenorhabditis elegans
C144I mutant with dramatically reduced GGT activity Homo sapiens
D331V little effect on GGT activity Caenorhabditis elegans
D392A little effect on GGT activity Homo sapiens
E371T mutant with reduced GGT activity Caenorhabditis elegans
E547K mutant with increased GGT activity Caenorhabditis elegans
F131L mutant with markedly reduced GGT activity Caenorhabditis elegans
F143L mutant with reduced GGT activity Homo sapiens
F372Y little effect on GGT activity Caenorhabditis elegans
H165S mutant with reduced GGT activity Caenorhabditis elegans
K482A mutant with increased GGT activity Caenorhabditis elegans
K674V no effect on GGT activity Caenorhabditis elegans
L124V mutant with 40% inhibited GGT activity Caenorhabditis elegans
L125V mutant with 70% inhibited GGT activity Caenorhabditis elegans
L136V mutant with increased GGT activity Homo sapiens
L137V mutant with increased GGT activity Homo sapiens
L192K mutant with reduced GGT activity Caenorhabditis elegans
L196I mutant with markedly reduced GGT activity Caenorhabditis elegans
L208I mutant with markedly reduced GGT activity Homo sapiens
L42K mutant with increased GGT activity Caenorhabditis elegans
L491M mutant with little increased GGT activity Caenorhabditis elegans
L562I mutant with increased GGT activity Caenorhabditis elegans
L591I mutant with increased GGT activity Caenorhabditis elegans
M406L mutant with reduced GGT activity Caenorhabditis elegans
M642L mutant with increased GGT activity Caenorhabditis elegans
M650L no effect on GGT activity Homo sapiens
M724A little effect on GGT activity Caenorhabditis elegans
additional information mutagenesis of aspartates of the DXD-like motif in LH3 eliminates GGT activity Homo sapiens
P591N no effect on GGT activity Caenorhabditis elegans
Q613E mutant with little increased GGT activity Caenorhabditis elegans
S204C mutant with reduced GGT activity Caenorhabditis elegans
V622L no effect on GGT activity Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-
soluble
-
 Caenorhabditis elegans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Homo sapiens posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Caenorhabditis elegans posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the amino acids important for the GGT activity are located in the N-terminal part of LH3/GGT protein separate from the C-terminal LH active site Homo sapiens ?
-
 ?
additional information lysyl hydroxylase and collagen glucosyltransferase activities are associated with the same gene product, the amino acids important for the GGT activity are located in the N-terminal part of LH/GGT protein separate from the C-terminal LH active site, C-132 and L-196 are important for the catalytic activity of GGT Caenorhabditis elegans ?
-
 ?
additional information multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase and collagen glucosyltransferase activities Homo sapiens ?
-
 ?
additional information C-144 and L-208 are important for the catalytic activity of GGT, DXD-like motif is required for activity, no GGT activity in LH1 and LH2 Homo sapiens ?
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Synonyms

Synonyms Comment Organism
More GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 Homo sapiens
More GGT activity is associated with lysyl hydroxylase, LH Caenorhabditis elegans
More cf. EC 2.4.1.50 Homo sapiens
More cf. EC 2.4.1.50 Caenorhabditis elegans