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Literature summary for 2.4.1.66 extracted from

  • Sricholpech, M.; Perdivara, I.; Yokoyama, M.; Nagaoka, H.; Terajima, M.; Tomer, K.B.; Yamauchi, M.
    Lysyl hydroxylase 3-mediated glucosylation in type I collagen: molecular loci and biological significance (2012), J. Biol. Chem., 287, 22998-23009.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.66

Cloned(Commentary)

Cloned (Comment) Organism
LH3-V5/His fusion protein is expressed in HEK-293 cells Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 x * 96000, LH3-V5/His fusion protein, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
LH3-V5/His fusion protein is purified using nickel-nitrilotriacetic acid-agarose resin column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen
-
 Mus musculus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Subunits

Subunits Comment Organism
? x * 96000, LH3-V5/His fusion protein, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
LH3
-
 Mus musculus
LH3
-
 Homo sapiens
lysyl hydroxylase 3
-
 Homo sapiens
lysyl hydroxylase 3 a multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities Mus musculus
PLOD3
-
 Mus musculus

General Information

General Information Comment Organism
malfunction an impairment of lysyl hydroxylase 3 function significantly affects type I collagen fibrillogenesis Mus musculus
malfunction lysyl hydroxylase 3-mediated glucosylation occurs at specific sites in collagen, including cross-linking sites, and suppression of this modification results in defective collagen and mineralization Homo sapiens
metabolism lysyl hydroxylase 3 functions as glucosyltransferase in type I collagen Homo sapiens
physiological function the lysyl hydroxylase 3-mediated glucosylation occurs at the specific molecular loci in the type I collagen molecule and plays critical roles in controlling collagen cross-linking, fibrillogenesis, and mineralization Homo sapiens
physiological function the major function of lysyl hydroxylase 3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen Mus musculus