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Literature summary for 2.4.1.67 extracted from

  • Gaudreault, P.R.; Webb, J.A.
    Stachyose synthesis in leaves of Cucurbita pepo (1981), Phytochemistry, 20, 2629-2633.
No PubMed abstract available
Search for more literature for 2.4.1.67

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect of EDTA Cucurbita pepo

Inhibitors

Inhibitors Comment Organism Structure
Ag+ weak Cucurbita pepo
Ca2+ weak Cucurbita pepo
Co2+
-
 Cucurbita pepo
Cu2+ weak Cucurbita pepo
Hg2+
-
 Cucurbita pepo
melibiose i.e. 6-O-alpha-D-galactosyl-alpha-D-glucose, non-competitive to raffinose, weak Cucurbita pepo
Mg2+ weak Cucurbita pepo
Mn2+
-
 Cucurbita pepo
additional information no inhibitors: 5 mM EDTA, 3 mM MoO42-, K+, Na+, Fe3+, stachyose, galactose Cucurbita pepo
myo-inositol strong, competitive Cucurbita pepo
Ni2+
-
 Cucurbita pepo
Tris buffer 0.1 M Cucurbita pepo
Zn2+
-
 Cucurbita pepo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 1-alpha-D-galactosyl-myo-inositol hydrolysis Cucurbita pepo
4.6
-
 raffinose + 1-alpha-D-galactosyl-myo-inositol Cucurbita pepo
5.2
-
 melibiose + 1-alpha-D-galactosyl-myo-inositol Cucurbita pepo
7.7
-
 1-alpha-D-galactosyl-myo-inositol + raffinose Cucurbita pepo

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Cucurbita pepo 5737
-

Organism

Organism UniProt Comment Textmining
Cucurbita pepo
-
 var. melopepo f. torticolis Bailey
-

Purification (Commentary)

Purification (Comment) Organism
partial Cucurbita pepo

Source Tissue

Source Tissue Comment Organism Textmining
leaf mature Cucurbita pepo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0185
-
 partially purified enzyme Cucurbita pepo

Storage Stability

Storage Stability Organism
4°C, 0.1 M sodium phosphate buffer, 20 mM 2-mercaptoethanol, up to 45 days Cucurbita pepo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-alpha-D-galactosyl-myo-inositol + H2O hydrolysis Cucurbita pepo myo-inositol + D-galactose
-
 ?
1-alpha-D-galactosyl-myo-inositol + melibiose 10% activity compared to raffinose Cucurbita pepo myo-inositol + alpha-D-galactosyl-6-O-D-galactosyl-alpha-D-glucose i.e. manninotriose ir
1-alpha-D-galactosyl-myo-inositol + raffinose i.e. galactinol Cucurbita pepo myo-inositol + stachyose
-
 r
additional information high donor specificity: UDP-galactose or melibiose are no galactosyl-donors Cucurbita pepo ?
-
 ?
additional information no substrates are sucrose, maltose, fructose, cellobiose, gentiobiose, melizitose, trehalose Cucurbita pepo ?
-
 ?
additional information no substrates: lactose, glucose, maltotriose, manninotriose Cucurbita pepo ?
-
 ?
p-nitrophenyl-alpha-D-galactopyranoside + raffinose about 10% activity compared to 1-alpha-D-galactosyl-myo-inositol Cucurbita pepo p-nitrophenol + stachyose
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cucurbita pepo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 6.9 plateau Cucurbita pepo

pH Range

pH Minimum pH Maximum Comment Organism
5.4 7.6 about half-maximal activity at pH 5.4 and pH 7.6 Cucurbita pepo

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
 myo-inositol
-
 Cucurbita pepo